结果与结论:①人髓核组织:miRNA-142-3p的表达由高到低的顺序为轻度退变组>中度退变组>重度退变组(P < 0.05),混合谱系激酶3、白细胞介素1β 基因与蛋白表达由低到高的顺序为:轻度退变组<中度退变组<重度退变组(P < 0.05),Ⅰ型胶原基因与蛋白表达由低到高的顺序为:轻度退变组<中度退变组<重度退变组...
Functionally, miRNA-142-3p inhibition may mitigate the degree of HUVEC senescence and improve HUVEC migration and proliferation. Mechanistically, SIRT1 was validated to be targeted by miRNA-142-3p in HUVECs. Moreover, SIRT1 inhibition reversed the effects of miRNA-142-3p inhibition on s...
miRNA142-3p targets Tet2 and impairs Treg differentiation and stability in models of type 1 diabetes Martin G. Scherm, Isabelle Serr, Adam M. Zahm, Jonathan Schug, Saverio Bellusci, Rossella Manfredini, Victoria K. Salb, Katharina Gerlach, Benno Weigmann, Anette-Gabriele Ziegler,...
方法分析miRNA-142-3p表达与非小细胞肺癌生存的相关性,采用Cox风险比例模型分析预后的独立影响因素.结果:miRNA-142-3p表达量与复发转移情况相关(P0.05),与患者性别,年龄,病理类型,分期及吸烟史无明显相关,生存分析显示miRNA-142-3p高表达组生存期明显低于低表达组(P0.01),Cox风险比例模型分析显示miRNA-142-3p表达可...
Furthermore, we show that upon metastasis to the brain, TRPA1 gets depleted, an effect triggered by the transfer of TRPA1-targeting exosomal microRNA (miRNA-142-3p) from brain astrocytes to cancer cells. This downregulation, in turn, inhibits TRPA1-mediated activation of FGFR2, hindering the...
目的探索miRNA-142-3p(miR-142-3p)对脂多糖(LPS)诱导的大鼠肺泡巨噬细胞炎症反应的负向调控作用及其可能机制.方法用100ng/mL LPS诱导肺泡巨噬细胞NR8383,实时定量PCR(qPCR)和蛋白质印迹法分别检测诱导后0,6,24,48h时细胞中miR-142-3p和高迁移率族蛋白(HMGB1)的表达.细胞体外转染miR-142-3p拟似物(miR-142-...
The mRNA expression of miRNA-142-3p as well as the mRNA and protein expression of mixed lineage kinase 3, interleukin-1尾, type I collagen, type II collagen in nucleus pulposus tissue were detected by qPCR and western blot assay. The correlation between the degree of human...
本发明涉及检测试剂盒技术领域,具体涉及miRNA1423p在预测IgA肾病治疗反应性中的应用.本发明首次发现,尿液miRNA1423p与IgA肾病患者治疗后完全缓解组及治疗后无完全缓解组具备相关性的.更具体地,本发明发现IgA肾病患者治疗后无完全缓解组尿液miRNA1423p表达量显著高于完全缓解组.并且,本发明采用miRNA1423p的相对表达量作...
AIM: To investigate the regulative effect of miRNA-338-3p (miR-338-3p) on cell growth in colorectal carcinoma (CRC). METHODS: The lentiviral vector pLV-THM-miR-338-3p and pLV-THM-miR-338-3p-inhibitor were constructed. The recombinant viral vector encoding the pre-miR-338-3p or miR-338...