Functionally, miRNA-142-3p inhibition may mitigate the degree of HUVEC senescence and improve HUVEC migration and proliferation. Mechanistically, SIRT1 was validated to be targeted by miRNA-142-3p in HUVECs. Moreover, SIRT1 inhibition reversed the effects of miRNA-142-3p inhibition on s...
The high efficacy and specificity of the miRNA inhibitor was demonstrated in several independent experimental settings. First, the inhibitor reduced miR142-3p abundance by >99% after 3 h of incubation (Supplementary Fig.6a). Second, application of the miRNA inhibitor significantly derepressed lucife...
结果与结论:①人髓核组织:miRNA-142-3p的表达由高到低的顺序为轻度退变组>中度退变组>重度退变组(P < 0.05),混合谱系激酶3、白细胞介素1β 基因与蛋白表达由低到高的顺序为:轻度退变组<中度退变组<重度退变组(P < 0.05),Ⅰ型胶原基因与蛋白表达由低到高的顺序为:轻度退变组<中度退变组<重度退变组...
Moreover, miRNA-142-3p mimic increased radiosensitivity in CD133+HUCB-MNC cells. Our results elucidated a novel regulation pathway in hematopoietic stem cells and suggested a potential therapeutic approach for blood system diseases therapy.doi:10.1038/s41598-018-23968-1Yuan, Fang...
Furthermore, we show that upon metastasis to the brain, TRPA1 gets depleted, an effect triggered by the transfer of TRPA1-targeting exosomal microRNA (miRNA-142-3p) from brain astrocytes to cancer cells. This downregulation, in turn, inhibits TRPA1-mediated activation of FGFR2, hindering the...
AIM: To investigate the regulative effect of miRNA-338-3p (miR-338-3p) on cell growth in colorectal carcinoma (CRC). METHODS: The lentiviral vector pLV-THM-miR-338-3p and pLV-THM-miR-338-3p-inhibitor were constructed. The recombinant viral vector encoding the pre-miR-338-3p or miR-338...
本发明的前期研究中,发现肝组织外泌体中miRNA-142a-3p在日本血吸虫感染肝组织外泌体(图1,图2,图3)中显著高表达(图4,图5),进一步确定其可以作为日本吸血虫诊断标志物,并以此建立日本吸血虫诊断试剂盒。 因此本发明要求保护血清外泌体miR-142a-3p作为日本血吸虫感染诊断标志物的应用,所述血清外泌体miR-142a-...
The mRNA expression of miRNA-142-3p as well as the mRNA and protein expression of mixed lineage kinase 3, interleukin-1尾, type I collagen, type II collagen in nucleus pulposus tissue were detected by qPCR and western blot assay. The correlation between the degree of human...
本发明涉及检测试剂盒技术领域,具体涉及miRNA1423p在预测IgA肾病治疗反应性中的应用.本发明首次发现,尿液miRNA1423p与IgA肾病患者治疗后完全缓解组及治疗后无完全缓解组具备相关性的.更具体地,本发明发现IgA肾病患者治疗后无完全缓解组尿液miRNA1423p表达量显著高于完全缓解组.并且,本发明采用miRNA1423p的相对表达量作...