Dicer is a central enzyme in microRNA (miRNA) processing. We identified a Dicer-independent miRNA biogenesis pathway that uses Argonaute2 (Ago2) slicer catalytic activity. In contrast to other miRNAs, miR-451 levels were refractory to dicer loss of function but were reduced in MZago2 (...
第一个靶向 miRNA 的小分子得追溯到 2008 年，Shan 等人通过荧光标签筛选 FDA 库，发现依诺沙星 (Enoxacin) 可增强 siRNA 介导的 mRNA 降解，能促进内源性 miRNAs 的生物发生，该作用依赖于 TRBP 途径。但因 miRNA 的特殊性，不少小分子都是靶向 AGO、TRBP 等调控 miRNA 生物发生的关键分子，而非直接靶向。...
“micro”“mi”是微小的意思,顾名思义,miRNA 就是小的非编码 RNA,长度约 23 个核苷酸 (nt),它在转录后的基因调控中发挥关键作用,包括疾病的发生、细胞分化与组织发育,细胞凋亡等等。 miRNA 的生物发生及在疾病中的作用 miRNA 从哪儿来:一般而言,在细胞核内,通过 RNA 聚合酶 Ⅱ 转录生成初始miRNA (pri-...
It is now becoming apparent that siRNAs and miRNAs are related molecules, sharing common processing pathways and maybe even functional mechanisms. Currently, most miRNA researchers are analyzing miRNA expression patterns by Northern blot, a technique that is relatively insensitive and labor in...
研究人员针对细菌感染急性应答期的miRNAs展开了系统性的分析,从中发现了一种称为miR-92d的小分子RNA,能调控这一脊索动物的补体途径(complement pathway)。 通过生物信息学分析,研究人员在白氏文昌鱼(Branchiostoma belcheri)的基因组中找到了155个潜在miRNAs,其中57个是之前从未被报道过的miRNAs(称为bbe-miRNAs),并且研...
a Schematic diagram of the general miRNA-processing pathway by core miRNA processors (Drosha/DGCR8 and Dicer/TARBP) and auxiliary factors (DDX23 and Lin28B). Depletion of core microprocessors Drosha and Dicer derepressed the firefly luciferase activity of luc-21-miRDREL and luc-let-7a-miRDREL...
27、 expression profiling in cancer from a bioinformatics prospective. Expert Rev Mol Diagn, 2007. 7(6): p. 787-92.4.Cullen, B.R., Transcription and processing of human microRNA precursors. Mol Cell, 2004. 16(6): p. 861-5.5.Peter, M.E., Let-7 and miR-200 microRNAs: guardians agai...
miRNA 生物功能 miRNA molecular biology TranscriptionalRegulationofEukaryoticGenes(真核基因的转录调控)1.Transcriptionalinitiation2.Histonemodification3.DNAmethylation Post-TranscriptionalRegulationofEukaryoticGenes(真核基因的转录后调控)•RNAsilencing(siRNAandmiRNA)•Proteindegradation Reference：GenesIX(BenjaminLewin)...
The classical miRNA biogenesis pathway and the associated proteins are discussed along with the emerging concept on the processing of miRNA-encoding introns (mirtrons). It also contains a concise account of plant miRNA targets and functions with focus on the recent successful attempt on engineering ...
In this review, we briefly summarize the pathway of miRNA precursor processing during miRNA biogenesis and continue with the description of the miRISC assembly process. Then, we present the miRNA-mediated mechanisms of gene expression regulation in detail, and we gather information concerning the ...