(e) Table categorizing validated miRNA-mRNA interactions based on involvement with multiple miRNAs. SD: standard diet; HFD: high-fat diet; miRNA: microRNA; qPCR: quantitative polymerase chain reaction. 2.7. Functional Analysis of Pathways and Leading-Edge Genes Targeted by DE miRNAs To identify ...
(EZBioscience, MN, USA), and RT-qPCR was performed on miRNA using miRNA Universal SYBR qPCR-MasterMix (Vazyme, Nanjing, China). The relative gene expression level was calculated using the 2−ΔΔCtmethod. The reaction conditions of mRNA and miRNA qPCR were pre-denaturation at 95 °C for...
Figure 2. Regulatory network of lncRNA–miRNA–mRNA compared to SS10. Differential analysis was conducted between the individual with the lowest volume of wood (SS10) and other nine samples by using SS10 as reference sample. Across nine comparison pairs, the three types of differential RNAs tha...
Since interactions between transcripts, including long non-coding RNA (lncRNA), microRNA(miRNA) and mRNA, regulate the pathophysiology of disease, we performed unbiased miRarray profiling in the heart of diabetic rabbits following I/R injury with/without RAPA treatment to identify differentially ...
cardiomyocyte; stem cell; cardiovascular biology; miRNA; cardiac differentiation; beta-adrenergic stress; target prediction; miRNA–mRNA interaction 1. Introduction Cardiovascular diseases (CVDs) are a major cause of disease burden in the world, with incidence rates on the rise [1]. Most therapy ...
2.5. Reconstruction of lncRNA–miRNA–mRNA ceRNA Regulatory Network To understand how ncRNAs (e.g., lncRNAs) regulate gene expression by targeting miRNAs and messenger mRNAs, using knowledge extracted from the STRING online database and other relevant papers via literature mining, a lncRNA–miRNA–...
the lncRNA–miRNA–mRNA interactions were investigated using high-throughput sequencing. A total of 6772 differentially expressed mRNAs (DEmRNAs), 2284 DElncRNAs, and 244 DEmiRNAs were obtained between gynogenetic female ovaries and sex-reversed neo-male testes. Genes in the steroid hormone biosynthe...