源于骨髓间充质干细胞的外泌体(MSC-Exos)包载miR-499能够有效靶向子宫内膜癌细胞,显著抑制其增殖、迁移和侵袭能力,同时促进细胞凋亡,这种递送系统通过保护miR-499,提高其生物利用度,增强了对抗肿瘤的效果。 产品名称 源于骨髓间充质干细胞的外泌体(MSC-Exos)包载miR-499 目录号 ML-定制 荧光 可定制Cy、FITC...
实验结果显示,转染 miR-499a-5p 模拟物可下调 GPX4 的表达,增加 Fe2+和 MDA 水平,诱导 HSCs 发生铁死亡,抑制其激活。 miR-499a-5p 靶向 ETS1 下调 Exos 处理的 HSCs 中的 GPX4:通过数据库预测和实验验证,研究人员发现 miR-499a-5p 可靶向转录因子 ETS1。双荧光素酶报告基因实验证实了 miR-499a-5p 与 ...
血清m i R-499-5p㊁铁调素20联合检测在急性心肌梗死诊断中的应用* 周云,镇海涛,郭芬,李军ә,魏家兴,彭艺灵 湖北科技学院医学部国家级全科医学实验教学示范中心,湖北咸宁437100摘要:目的探讨血清微小R N A(m i R N A)-499-5p和铁调素(H e p c)-20联合检测对急性心肌梗死(AM I)的诊断价值㊂方法...
499表达水平与血清HMGB1含量呈负相关(P <0.05)㊂模型组小鼠颈动脉粥样硬化斑块中miR-499表达水平低于对照组,HMGB1表达水平高于对照组;miR-499+模型组小鼠颈动脉粥样硬化斑块病理改变较miR-NC +模型组改善,miR-499表达水平高于miR-NC +模型组,HMGB1表达水平低于miR-NC +模型组(P <0.05);Lenti-HMGB1+miR-499...
We found that both the α- and β-isoforms of the calcineurin catalytic subunit are direct targets of miR-499 and that miR-499 inhibits cardiomyocyte apoptosis through its suppression of calcineurin-mediated dephosphorylation of dynamin-related protein-1 (Drp1), thereby decreasing Drp1 accumulation in...
《miR-499-5p对猪肌纤维类型转化的调控及其机制研究》是黄志清为项目负责人，四川农业大学为依托单位的面上项目。项目摘要 肌纤维类型与猪肉品质密切相关。对比猪不同类型骨骼肌中miR-499-5p的表达水平，我们发现比目鱼肌（慢肌）显著高于趾长伸肌（快肌）。而Sox6的表达水平则相反。分析发现，猪Sox6 3'UTR中含有...
1.一种在有需要的受试者中治疗病理性心脏肥大、心肌梗死、或心力衰竭的方法,包括对所述受试者施用miR-208a或miR-208b的抑制剂和miR-499的抑制剂,其中miR-208a或miR-208b和miR-499的表达或活性在施用后的所述受试者的心脏细胞中降低。 2.权利要求2的方法,其中所述miR-208a或miR-208b的抑制剂和所述miR...
Deriving cell populations from human embryonic stem cells (hESCs) for cell-based therapy is considered a promising strategy to achieve functional cells, yet its translation to clinical practice depends on achieving fully defined differentiated cells. In
Jian-Xun Wang et al. show that mitochondrial fission, which occurs during cell death, is regulated in cardiomyocytes by the microRNA miR-499 through a mechanism involving the phosphatase calcineurin and its substrate Drp1. Overexpression of miR-499 was a