miR-494-3p miRNA表达差异最高的miRNA(Fig.5C); 在A549中过表达miR-494-3p,并通过流式细胞仪检查SP细胞区域的百分比。与对照相比,较高水平的miR-494-3p导致SP的显著增加(Fig.5D-E); 使用miR-494-3p抑制剂转染A549,降低其表达后,与对照相比并不影响SP比...
《MSC通过miR-494调控M2型巨噬细胞转化影响子痫前期的机制研究》是依托南京大学，由王志群担任项目负责人的面上项目。项目摘要 子痫前期（PE）是妊娠常见并发症，我国孕产妇死亡的第二原因。但发病机制未明，且缺乏有效治疗手段。免疫失调被认为是PE的发病机制之一,我们发现间充质干细胞（MSC）能够在母...
目的探讨血清微小RNA-494(miR-494)表达水平对心脏术后急性肾损伤(AKI)患儿的预后预测价值。 方法选择2016年1月至2019年3月三亚市人民医院收治的116例先天性心脏病(先心病)体外循环手术后并发AKI的患儿。所有患儿均于术后采用实时荧光定量聚合酶链反应(RT-qPCR)检测血清miR-494表达,用酶联免疫吸附试验(ELISA)检测...
& He, J. miR-494 suppresses tumor growth of epithelial ovarian carcinoma by targeting IGF1 R. Tumor Biology. 2016;37:7767–7776. doi: 10.1007/s13277-015-4603-8 The authors contacted the editorial office to request a retraction. According to them, they are not able to reproduce the ...
MiR-494 induces metabolic changes through G6pc targeting and modulates sorafenib response in hepatocellular carcinoma Christian Bergamini, Ilaria Leoni, Nicola Rizzardi, Mattia Melli, Giuseppe Galvani, Camelia Alexandra Coada, Catia Giovannini, Elisa Monti, Irene Liparulo, Francesca Valenti, ...
此外,沉默miR -377-3p/miR-494-3p及其相应的抑制剂在mRNA和蛋白水平上显著提高了DMS273和H82细胞中IGF1R的表达(Fig.5F, H和Fig.6A)。相反,miR-377-3p和miR-494-3p及其模拟物过表达显著降低了SCLC细胞中IGF1R的mRNA和蛋白水...
www.nature.com/scientificreports OPEN MiR-494 acts as a tumor promoter by targeting CASP2 in non-small cell lung cancer Received: 17 July 2018 Accepted: 24 January 2019 Published: xx xx xxxx Qiao Zhang1,Yan Li1, Mei Zhao1, Hong Lin1, Wenjie Wang1, Dongdong Li1, Wei...
目的探讨miR-494对人骨肉瘤细胞MG-63增殖与侵袭的影响,并验证Sox9是否为miR-494的靶基因.方法过表达miR-494后,利用CCK-8,克隆形成实验检测MG-63细胞的增殖,利用Transwell实验检测细胞的侵袭能力.Western blot与荧光定量PCR检测Sox9蛋白与m RNA水平的表达.结果 MG-63细胞转染腺病毒miR-494后,miR-494表达明显升高(t...
miR-377-3p (b) or miR-494-3p (c). 293T cells were co-transfected with a dual-luciferase reporter vector containing the putative binding sites/ mutated sequences for IGF1R and miR-377-3p/miR-494-3p, miR-377-...