探究MiR-367通过Wnt/β-连环蛋白(β-catenin)通路对成骨细胞增殖的抑制作用。方法 培养骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)后转染miR-367模拟物或抑制物,茜素红染色观察成骨分化;培养新生大鼠成骨细胞,转染miR-367模拟物或抑制物、联合使用β-catenin激动剂SKL2001或抑制剂XAV939,采用MTT...
在本项研究中,我们通过检测位于miR-367靶基因RYR33'UTR结合区域的多态位点rsl044129的基因型,来探究钙离子释放通道基因RYR33'UTR的SNP是否与乳腺癌的发病风险性、乳腺癌钙化和预后相关。从功能上验证miR-367与靶基因是否结合,rsl044129多态位点是否能影响miR-367对RYR3的调控,探究RYR3在乳腺癌细胞中发挥的重要作用和...
水稻线粒体基因组--序列多态性和基因组进化,以及线粒体、叶绿体和细胞核基因组间的基因迁移 热度: 三种鳜属鱼类GH基因的序列及多态性研究 热度: miR 367在RYR3基因靶序列的多态性影响乳腺癌的发病风险钙化和预后,miR 367在RYR3基因靶序列的多态性影响乳腺癌的发病风险钙化和预后,miR...
Thus, the miR-367/FBXW7 axis may be involved in the development and progression of NSCLC and may be valuable as a therapeutic target for the treatment of human NSCLC, especially cancers with high invasive potential. 展开 关键词: miR-367 proliferation invasion FBXW7 NSCLC ...
将包含miR-367-3p结合位点的circ-TLK1的野生型序列(WT-circ-TLK1)或不具有miR-367-3p结合位点的突变体(MUT-circ-TLK1)插入pmirGLO载体,将质粒与miR-367-3p mimics或miR-NC共转染至SK-N-SH细胞,采用双荧光素酶标记试剂盒考察...
FULL PAPER British Journal of Cancer (2015) 112, 1367–1375 | doi: 10.1038/bjc.2015.102 Keywords: pancreatic cancer; miR-367; Smad7; metastasis; epithelial-to-mesenchymal transition miR-367 promotes epithelial-to-mesenchymal transition and invasion of pancreatic ductal adenocarcinoma cells by ...
Mechanically, we identified that SNHG4 or MYO1B could bind with miR-367-3p by the luciferase reporter assays. Furthermore, the rescue experiments showed that the inhibition of miR-367-3p or the expression of MYO1B partially rescue the inhibition effects of SNHG4 in glioma cells. Our study ...
目的 探究长链非编码RNA(lncRNA)MEG8通过调控miR-367-3p/磷酸酶张力蛋白同源物基因(PTEN)介导慢性阻塞性肺疾病(COPD)发展的分子机制.方法 采用实时荧光定量聚合酶链反应(qPCR)检测16HBE细胞和COPD组织lncRNA MEG8表达水平;在香烟烟雾提取物(CSE)刺激后的16HBE细胞中过表达lncRNA MEG8及加入miR-367-3p inhibitor后...
Bailey, S., Ferraresso, M., Alonso-Crisostomo, L.et al.Correction: Targeting oncogenic microRNAs from the miR-371~373 and miR-302/367 clusters in malignant germ cell tumours causes growth inhibition through cell cycle disruption.Br J Cancer129, 1524 (2023). https://doi.org/10.1038/s41416...