设计研发了多功能Bi@PP/miR339纳米体系,其具有pH 响应性、缓解乏氧及CT 成像的特性;系统地阐述了miR339通过抑制靶基因USP8表达,进而抑制肿瘤细胞干性机制;证实Bi@PP/miR339可以实现食管癌放疗增敏,有望为临床食管癌治疗提供一种新的治疗模式(图1)。
乳腺癌细胞中miR-339—5p对BCL-6表达的调节
miR-339-5p：miRNA 339 5' 端的成熟序列 miRNA 往往一个家族（同一个数字）存在多个loci，它们成熟序...
长曲线与平皿克隆形成,观测miR-339-3p对GES-1细胞增殖的影响。 5、Western-blotting方法,检测miR-339-3p高表达对JAK3/STAT3信 号通路的影响。 结果:1、生物信息学分析,发现miR-339-3p与SOD3基因存在 相互作用;实时荧光定量PCR检测结果,显示miR-339-3p在GES-1 ...
目的 观察人胃癌细胞株及正常人胃黏膜上皮细胞株中miR-339-3p和miR-339-5p的表达水平,通过功能获得型(gain of function)实验验证miR-339-3p和miR-339-5p与胃癌的相关性,并阐明二者在肿瘤发生中的意义。 方法 采用SYBR GreenⅠ嵌合荧光法实时定量PCR技术分别检测人正常胃黏膜上皮细胞GES-1,胃癌MKN-45、SGC-79...
乳腺癌细胞中miR一339—5p对BCL一6表达的调节 刘 雪,吴正升,吴 强 摘要:目的 预测和验证miR-339-5p下游直接靶基因。方法 利用生物信息学方法预测miR-339-5p潜在靶基因;克隆目标基 因3’UTR,将其与含荧光素酶报告基因的质粒psiCHECK-2连接后,与miR一339-5pmimics共转染乳腺癌细胞株T47D,做荧光 ...
货号:cgr-miR-339 原价:￥4030 现价:￥3098.38节省:￥931.62 规格:1 kits 说明:cgr-miR-339 特异性茎环引物试剂盒 ￥615 产品参数 ---说明书下载--- microRNAs(miRNAs)是一类长度约19~24nt的非编码单链RNA,通过碱基互补配对的方式与靶基因的29’UTR区部分或完全互补,剪切靶基因的转录产物或者抑制转录产物的...
The expression of miR-339-3p was detected using quantitative real-time PCR. The levels of TNF-α, IL-1β, and IL-6 were detected using enzyme-linked immunosorbent assay. Cell apoptosis was measured using flow cytometry. The protein expressions of TNF receptor-associated factor 3 (TRAF3), ...
reported that miR-339-3p decreased cell inflammation and cell apoptosis in mice with AP through Anxa3 by activation of the Akt/mTOR signaling pathway [21]. However, the regulatory mechanism of miR-339-3p has not yet been fully discovered. Therefore, we investigated the role and regulatory ...
Consequently, overexpression of miR-339-5p positively impacts on p53-governed cellular responses such as proliferation arrest and senescence, whereas inhibition of miR-339-5p function perturbs the p53 response in cancer cells, allowing an increased proliferation rate. In addition, miR-339-5p ...