microRNA(miRNA)是一类内源性的非编码RNA,广泛参与细胞的生长发育、分化、增殖和凋亡等生物学过程.为了研究miR-let-7a对猪(Susscrofa)甲状腺的生长发育及甲状腺细胞凋亡的调控作用,本研究利用免疫荧光技术鉴定了大白猪的甲状腺组织及体外培养的甲状腺细胞,利用实时荧光定量PCR(qRT-PCR)检测了miR-let-7a在不同生长阶段...
MiR-let-7a/f-knockdown and CCR7-overexpression cell models were used to investigate the involvement of miR-let-7a/f-CCR7 signaling in the anti-metastatic effects of SLE. Results It was found that SLE upregulated levels of miR-let-7a/f in B16F10 melanoma tissues. SLE significantly elevated ...
目的:构建Ⅲ型转化生长因子β受体(TGFβR3)基因3'非编码区(3'UTR)双荧光素酶报告载体,并分析其与miR-let-7a的靶向关系.方法:根据micro RNA靶基因预测软件targetscan获取miR-let-7a与TGFβR3基因3'UTR潜在的互补结合位点;PCR扩增出TGFβR3基因3'UTR序列并克隆至pGEM-T载体,定点突变潜在互补结合位点后双酶切pGEM...
hsa-let-7a 6 22, 23, 24 2.30E−03 1.66 8.80E+03 5.30E+03 hsa-miR-23a 7 25 5.80E−03 1.61 1.00E+04 6.30E+03 hsa-miR-199a-5p 8 18, 19 1.30E−02 1.61 7.50E+03 4.60E+03 hsa-miR-126 9 26 1.50E−02... HOSHEN, Moshe,BRENNER, Baruch 被引量: 0发表: 0年来...
结果发现,TGF-β能够通过SMAD2/3诱导MIR100HG这一长链非编码RNA(在这一lncRNA的内含子中含3个miRNA,分别是miR-100, miR-125b和let-7a)在转录水平激活。有趣的是,他们发现,虽然miR-100和miR-125b的表达量随着TGF-β的处理时间而增加,而let-7a的表达量并不发生显著变化。进一步的研究发现,TGF-β同时诱导了...
There are comments on PubPeer for publication: Low expression of miR-let-7a promotes cell growth and invasion through the regulation of c-Myc in oral squamous cell carcinoma (2020)
miR-125b and let-7a in its intron, via SMAD2/3. Interestingly, we find that although the pro-tumourigenic miR-100 and miR-125b accordingly increase, the amount of anti-tumourigenic let-7a is unchanged, as TGF-β also induces LIN28B inhibiting its maturation. Notably, we demonstrate tha...
Ectopic expression of let-7a and miR-17-5p caused a noticeable reduction in PD-L1 levels in TNBC cells. In order to investigate the whole ceRNA circuit regulating PD-L1 in TNBC, intensive bioinformatic studies were performed. The lncRNA, Colon Cancer-associated transcript 1 (CCAT1), was ...
The plasma expression of the miR-21, miR-155, and miR-10b was significantly increased and the Let-7a plasma expression decreased in the breast cancer patients compromised to the control ones. There was a similar expression pattern of the miRs between the tissue and plasma samples. The plasma ...
Uterine miR-877-3p and let-7a-5p are increased during simulated menstruation in a mouse model 来自 科研支点 喜欢 0 阅读量: 16 作者:M Watters,CA Walker,AA Murray,M Nicol,JA Maybin 摘要: Lay summary Heavy periods are common and debilitating, but we do not fully understand how they are ...