目的 研究miR-542-3p在肝癌组织和细胞系中的表达情况,探讨miR-542-3p/survivin对肝癌细胞增殖和肿瘤生长影响的机制,以期为肝癌提供新的潜在治疗靶点.材料和方法 1.收集肝细胞癌患者的手术标本,测定miR-542-3p在肝癌组织及癌旁正常肝脏组织中的表达情况并分析miR-542-3p的不同表达水平对患者总体生存的影响.2.检测...
EMT是肝癌转移的关键过程,本研究中Western boltting和免疫荧光实验结果均提示,当miR-542-3p过表达时,上皮标志物E-cadherin蛋白表达增加,而间质标志物Vimentin、N-cadherin、Snail1蛋白表达减少,证明了miR-542-3p具有抑制肝癌细胞EMT的作用,结果提示miR...
同样,我们的研究发现CIRC_PTN在耐DDP的GBM细胞中可以与miR-542-3p相互作用,而miR-542-3p在耐DDP的GBM细胞中表达下调。 值得注意的是,PIK3R3可以触发PI3K/AKT信号通路的激活。此外,PI3K/AKT信号通路的激活也参与了乳腺癌细胞对DDP的耐药。MALAT1通过激活PI3K/AKT通路促进宫颈癌顺铂耐药。另外,以前的研究表明,CircA...
When the miR‐542‐3p binding site in sFRP1 3'UTR was deleted, it did not affect its expression. Western blot results showed that miR‐542‐3p inhibited the expression of SFRP1 protein. The expression of SFRP1 was significantly increased in osteoblast‐induced mesenchymal stem cells (MSC), ...
cgr-miR-542-3p qPCR试剂盒 分类:microRNA qPCR Kits 品牌:BioTNT 货号:cgr-miR-542-3p 原价:￥4030 现价:￥3098.38 节省:￥932 规格:1 kits 说明:cgr-miR-542-3p 特异性茎环引物试剂盒 ￥615产品参数 ---说明书下载--- microRNAs(miRNAs)是一类长度约19~24nt的非编码单链RNA,通过碱基互补配对的方...
When co-transfected with miR-542-3p mimic and CTTN overexpression vector, the inhibitory effect of miR-542-3p was blocked. This indicates that miR-542-3p regulates CTTN in a targeted manner to modulate the growth and invasion of colorectal cancer cells. The present study thus provides new ...
PURPOSE: In this study, miR-542-3p appended SRF/ATRA-loaded solid lipid nanoparticle was successfully prepared and demonstrated for its therapeutic efficacy against gastric cancers. METHODS: The particles were nanosized and typically spherical in shape. In vitro release study showed that release of ...
Firstly, we determined the expression of miR-542–3p and glutathione peroxidase 5 (GPx5) in the epididymis of young and adult goats using RT-qPCR assay, and found that miR-542–3p and GPx5 exhibited inverse expression levels. Our results showed that the expression level of miR-542–3p in...
Autophagy has recently been shown to be critical for protecting peripheral nerve regeneration. This study explored the impact of miR-542-3p on diabetic corneal nerve regeneration and epithelial healing through the regulation of autophagy. A type 1 diabet
2.1. Downregulation of miR-542-3p in Bones of MTX-Treated Rats To determine if miR-542-3p is differentially expressed in bones of MTX-treated rats, its expression levels were measured using RT-qPCR in tibial bone samples (containing cortical and trabecular bones of the metaphyseal region with ...