通过双荧光素酶报道实验和WesternBlot方法验证miR-519d对OCT4的靶向调控。利用OCT4特异性干扰RNA证实miR-519d通过靶向调控OCT4抑制膀胱癌细胞系5673增殖。结果:在膀胱癌细胞系过表达miR-519d后,细胞活性、增殖能力明显下降;双荧光素酶报道实验结果表明,相对于各对照组,共转染OCT43UTR载体与miR-519d组荧光素酶
MiR-519d增强细胞迁移,粘附,体外侵袭和体内肺转移。 EphA4是miR-519d的直接靶标。 EphA4是miR-519d在黑素瘤进展中的功能重要靶基因。 EphA4的恢复抑制miR-519d介导的ERK活化,表皮 - 间质转化和体内肺转移。 相互作用的miR-519d上调和EphA4下调与黑素瘤患者的转移相关。 来源:miR-519d Promotes Melanoma Pr...
Human MIR519D qPCR Primer Pair,即人MIR519D qPCR引物对,主要用于基于SYBR Green的qPCR、One-Step qRT-PCR或semi-quantitative PCR。本引物为预先设计、经过qPCR验证、预混的引物对。 qPCR (Quantitative PCR)即定量PCR,也称实时荧光定量PCR或实时定量PCR (Real-time quantitative PCR)、实时PCR (Real-time PCR)...
Western blot结果显示,与NC-LV组相比,OE-miR-519d-LV组和OE-miR-519dLV+pcDNA3.1-HRE3组MMP-2、MMP-9、N-cadherin和Vimentin蛋白表达降低(P<0.05),而E-cadherin表达升高(P<0.05);与OE-miR-519d-LV组相比,OE-miR-519d-LV+pcDNA3.1-HRE3组MMP-2、MMP-9、N-cadherin和Vimentin蛋白表升高(P<0.05),而E...
miR-519d-3p 和 BMP2 对骨诱导的调节:miR-519d-3p 过表达抑制 MC3T3-E1 细胞成骨分化标记物的表达(P<0.001),降低细胞活力(P<0.001),上调促凋亡因子 Bax 和 Cleaved-caspase-3 mRNA 表达,下调抗凋亡因子 Bcl-2 表达(P<0.001),促进细胞凋亡(P<0.001)。而 BMP2 过表达能在一定程度上抵消 miR-519d-3p...
内容提示: 中国现代医生2018年7月第56卷第21期 基础医学 · miR-5 19d靶向CDKNIA/p2 1调控宫颈癌细胞 增殖的初步研究 刘洁曾烨 周珏宇 陈志超 张汉荣 赖姝或 昊小丽 谭令梅 1.南方医科大学南方医院妇产科 ,广东广州 510515;2.南方 医科大学南方医院 口腔科 ,广东广州 3.南方医科大学基础医学院生物化学与分子...
Guangrao County People's Hospital, Dongying City, Shandong Province 257300, People's Republic of China Background: MiR-519d and miR-328-3p had tumor-regulatory properties in different cancers, but their combinatorial effects and potential common target in breast cancer had not been fully reported....
Therefore, the present study assessed the expression level of miR-519d-3p in cervical cancer cells and examined the effects of miR-519d-3p overexpression on DDP resistance in cervical cancer cells. Moreover, the present study analyzed the potential regulatory mechanism of the miR-519d-3p/HIF-...
MicroRNAs (miRNAs) play pivotal roles in the development of various cancer types, including cervical cancer. In this study, we showed that miR-519d, a miRNA within the chromosome 19 miRNA cluster, was significantly upregulated in cervical cancer tissues,
miR-519d downregulates LEP expression to inhibit preeclampsia development. Open Med. 2021;16(1):1215–27. DOI: 10.1515/med-2021-0244. This article has been retracted due to possible irregularities in the data images presented in results discovered by author. His team is working currently on ...