目的:构建野生型及突变型HMGA2基因3'-非编码区(3'-UTR)的双荧光素酶报告基因载体,验证miR-33b-5p与HMGA2之间的靶向关系.方法:生物信息学相关软件预测miR-33b-5p与HMGA2之间的靶向关系;PCR扩增HMGA2的基因片段,构建HMGA2野生型和突变型双荧光素酶报告载体;将miR-33b-5p和阴性对照物分别与野生型GV272-HMGA2-...
货号:chi-miR-33b-5p 原价:￥4030 现价:￥3098.38节省:￥931.62 规格:1 kits 说明:chi-miR-33b-5p 特异性茎环引物试剂盒 ￥615 产品参数 ---说明书下载--- microRNAs(miRNAs)是一类长度约19~24nt的非编码单链RNA,通过碱基互补配对的方式与靶基因的29’UTR区部分或完全互补,剪切靶基因的转录产物或者抑制转录...
Conclusion: Downregulation of MCF2L-AS1 inhibits the proliferation, migration and invasion and promotes apoptosis of gastric cancer cells by upregulating miR-33b-5p; MCF2L-AS1 participates in the malignant biological behavior of gastric cancer cells by targeting th...
胎儿生长受限外泌体miR-33b-5pIGF2BP1脐静脉内皮细胞PI3K/AKT信号通路目的:胎儿生长受限(fetal growth restriction,FGR)又称宫内生长受限(intrauterine growth restriction,IUGR),是指胎儿在宫内未达到其应有的生长水平,此疾病易引起妊娠期胎儿窒息或胎死宫内,并影响远期成人后的长期健康.本实验通过分离及鉴定生长受限...
miR-33b-5p胃癌增殖迁移侵袭凋亡目的:探讨lncRNA MCF2L-AS1对胃癌细胞恶性生物学行为的影响及分子机制.方法:选取45例胃癌患者的癌组织及癌旁正常组织,或培养胃黏膜上皮细胞GES-1,胃癌细胞HGC-27,采用RT-qPCR检测MCF2L-AS1和miR-33b-5p的表达水平.采用双荧光素酶报告实验检测MCF2L-AS1和miR-33b-5p的靶向关系....
Conclusion: Curcumin inhibits SIRT6 expression via upregulating miR-33b-5p expression, which leads to the inhibition of OS cell migration and invasion. Thus, curcumin is a potential therapeutic agent for the management of OS.doi:10.4314/tjpr.v18i12.4Hang Lin...
miR-33b-5pSIRT6cell survivalLong non-coding RNAs (lncRNAs) are emerging as vital regulators in various physiological and pathological processes. It was recently found that lncRNA HIF1A-AS2 could play oncogenic roles in several cancers. However, the function and regulatory mechanism of lncRNA HIF...