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目的 研究miR-32-5p对子宫内膜癌细胞迁移和侵袭的影响和潜在机制.方法 以人正常子宫内膜上皮细胞hEEC为对照,qRT-PCR和Western blot检测子宫内膜癌细胞系Ishikawa,HEC-1A和JEC中miR-32-5p和FOXN3的表达;在Ishikawa细胞中高表达FOXN3和敲减miR-32-5p,Western blot检测FOXN3,E-Cadherin,N-Cadherin和Vimentin蛋白表达...
12∙ 临床研究 ∙DOI: :10.3872/j.issn.1007-385x.2019.12.011lncRNA XIST 通过 miR-32-5p/EZH2 分 子轴调控结直 肠癌 HCT-8细胞的恶性生物学行为吴瑞平,陈冠阳,陈志康(中南大学湘雅医院 直肠肛门外科,湖南 长沙 410008)[摘 要] 目的: 探讨lncRNA XIST通过miR-32-5p/果蝇Zeste基因增强子同源物2 (...
Similarly, miR-32-5p knockdown alleviated cytokine production in lipopolysaccharide (LPS)-treated spinal microglial cells, whereas its overexpression had the opposite effect. Mechanistic investigations revealed Dual-specificity phosphatase 5 (Dusp5) as a direct target of miR-32-5p, which is involved in...
Objective To investigate the correlation between circulating exosomal miR-485-3p, miR-32- 5p expression, and the incidence of early onset coronary heart disease (CHD). Methods This study retrospectively included 50 patients with a confirmed diagnosis of early onset...
方法将6~8周龄动脉粥样硬化雄性ApoE^(-/-)小鼠随机分为实验组和模型组,实验组通过给小鼠注射miR-32-5p慢病毒构建过表达模型,模型组注射同等剂量生理盐水.4周后解剖小鼠,留取主动脉组织常规石蜡切片,采用苏木精-伊红(hematoxylin and eosin staining,HE)染色,免疫组化,免疫荧光法分别检测主动脉斑块面积及斑块内巨...
i R-32-5p水平明显低于死亡组(P<0.05),而血清m i R-409-3p水平明显高于死亡组(P<0.05)㊂血清m i R-32-5p㊁m i R-409-3p水平诊断宫颈癌的效能明显优于β-H C G,m i R-32-5p和m i R-409-3p联合检测诊断和预测3年内出现死亡的效能明显优于单项指标㊂结论 m i R-32-5p㊁m i R...
货号:cgr-miR-32-5p 原价:￥4030 现价:￥3098.38节省:￥931.62 规格:1 kits 说明:cgr-miR-32-5p 特异性茎环引物试剂盒 ￥615 产品参数 ---说明书下载--- microRNAs(miRNAs)是一类长度约19~24nt的非编码单链RNA,通过碱基互补配对的方式与靶基因的29’UTR区部分或完全互补,剪切靶基因的转录产物或者抑制转录...
结果 食管癌组织中的miR-32-5p和IL-6表达量显著高于正常食管组织(P<0.05),KLF4表达量显著低于正常食管组织(P<0.05);抑制miR-32-5p表达和过表达KLF4均可抑制Eca-109细胞增殖、迁移和侵袭,抑制炎症因子IL-6的产生(均P<0.05);miR-32-5p靶向负调控KLF4的表达;抑制KLF4表达可逆转抑制miR-32-5p对Eca-109细...
Vascular calcification is often associated with chronic inflammation and is a risk factor for brain arterial stiffness. Our previous results showed that miR32-5p was positively correlated with vascular smooth muscle cells (VSMC) calcification, but it is