结论 miR-3074-5p可能通过直接靶向抑制p27的表达而参与调控人绒毛外滋养层细胞的生理活性,进而影响胎盘发育及功能;胎盘组织中miR-3074-5p的异常低表达可能通过诱导p27的表达而参与子痫前期的病理过程。【关键词】 miR-3074-5p; p27; 子痫前期; 胎盘; 滋养层细胞基金项目:国家自然科学基金(81701445);天津市自然科学...
但本研究结果显示,在子痫前期患者的胎盘组织中,miR-3074-5p的表达水平显著下调,而p27蛋白的表达水平则显著增高,两者之间呈负相关,并且通过双荧光素酶报告基因检测系统证实 miR-3074-5p能通过与p27 mRNA 3’UTR结合而抑制其表达,提示胎盘组织中...
miRNAmiR-3074-5p目的本研究旨在通过检测类风湿关节炎外周血单个核细胞(peripheral blood mononuclear cells,PBMCs)中TNFSF14(tumor necrosis factor superfamily 14)表达情况,寻找其上游的miRNA,并探讨它们参与RA的可能机制.方法我们通过RT-PCR检测了TNFSF14在22例RA和22例健康人(HCs)PBMCs中的mRNA表达,通过生信分析...
本发明涉及miR30745p的新用途,miR30745p可作为microRNA分子标志物在制备类风湿关节炎的诊断试剂或试剂盒中进行应用.在miR30745p抑制表达组中TNFSF14的mRNA表达水平明显高于对照组,通过计算受试者工作特征曲线(ROC)和斯皮尔曼相关性检验评估miRNA在RA中的诊断价值,确认其在RA诊断中的潜在作用.miR30745p可以被认为是...
本发明涉及miR30745p的新用途,miR30745p可作为microRNA分子标志物在制备类风湿关节炎的诊断试剂或试剂盒中进行应用.在miR30745p抑制表达组中TNFSF14的mRNA表达水平明显高于对照组,通过计算受试者工作特征曲线(ROC)和斯皮尔曼相关性检验评估miRNA在RA中的诊断价值,确认其在RA诊断中的潜在作用.miR30745p可以被认为是...
Up-regulated miR-3074-5p expression led to the significant decreased GDF15 expression in HTR8/SVneo cells, and this effect could be efficiently reversed by the overexpression of EIF2S1. Meanwhile, the suppressive effects of miR-3074-5p on proliferation, migration, and invasion of HTR8/SVneo ...
miR-3074-5pAs the population ages, the prevalence of atherosclerosis (AS), a significant cause of cardiovascular disease (CVD), continues to increase. Apoptosis is an independent risk factor for atherosclerosis. Macrophages are the primary immune cell group in AS lesions, and their apoptosis plays...
Either miR-3074-5p overexpression or ERα knockdown promoted transcriptional activity of NF-κB/p65, induced M1polarization and pyroptosis of THP1-derived Ms, accompanied with increased intracellular levels of cleaved Caspase-1, cleaved IL-1β, NLRP3, cleaved GSDMD and ASC aggregation. Furthermore,...
结果与对照组相比,子痫前期组胎盘组织中miR-3074-5p(P=0.034)与CCND1蛋白的表达量都显著下调(P=0.031),而p27蛋白的表达量则显著上调(P=0.010);IHC结果显示,p27和CCND1蛋白主要表达于合体滋养层细胞中.双荧光素酶报告系统检测结果证实,miR-3074-5p能与p27 mRNA的3'UTR序列结合而抑制其表达;敲低HTR-8/SV...
qRT-PCR was used to determine the expression of miR-3074-5p in MC3T3-E1 cells under iron overload. The proliferation of MC3T3-E1 cells was tested using CCK-8 assays, and apoptosis was measured using flow cytometry. The miRanda and TargetScan databases were used to predict the target genes ...