The anti-inflammatory role ofmiR-23b-3p (miR-23b) is known in autoimmune diseases like multiple sclerosis, systemic lupus erythematosus, and rheumatoid arthritis. However, its role in sepsis-related acute lung injury (ALI) and its effect on macrophages in ALI remain unexplored. This investigation ...
为了分析血液外胞体miRNA对延边黄牛垂体细胞中生长激素(GH)分泌的影响,试验选择在延边黄牛和韩延牛血液外胞体中存在显著差异表达的miR-23b-3p,分析其对延边黄牛垂体细胞中GH分泌的影响机制.首先进行延边黄牛垂体细胞的原代培养,然后将miR-23b-3p的mimics(miR-23b-3p-mi组),mimics对照品(NC组),inhibitor(miR-23b-3p...
miR-23b-3p mimics were purchased from GenePharma (Shanghai, China). RNA extraction and quantitative real-time PCR Trizol reagent (Sigma, USA) was used to extract total cellular RNA containing miRNA from cultured cells. SYBR® Premix Ex Taq™ kit (Takara Bio, Japan) was used to quantify ...
(E and F) GC cells were transfected with miR-23b-3p mimics, inhibitor or NC, and the expression of ATG12 and HMGB2 was determined using qRT-PCR and western blot analysis. (G) Dual luciferase assays were performed in SGC7901/VCR cells after cotransfection with wild-type or mutant ATG12...
(E and F) GC cells were transfected with miR-23b-3p mimics, inhibitor or NC, and the expression of ATG12 and HMGB2 was determined using qRT-PCR and western blot analysis. (G) Dual luciferase assays were performed in SGC7901/VCR cells after cotransfection with wild-type or mutant ATG12...
Increasing miR-23b-3p mimics diminished RCOR1's mRNA and protein levels, while raising miR-23b-3p levels boosted RCOR1's expression. Moreover, the stimulatory impact of RCOR1 on prostate cancer cell development could be countered by elevating miR-23b-3p mimics. Conclusion: In summary, our ...
采用脂质体转染技术将miR-23b-3p mimics,mimics-NC转染至SW620细胞,采用Transwell实验和划痕实验检测其侵袭和迁移能力的改变.利用生物信息学软件预测并通过双荧光素酶报告基因实验验证miR-23b-3p和KLF3的结合位点.将KLF3过表达质粒(pcDNA3.1-KLF3)或空载质粒(Vector)单独或联合miR-23b-3p mimics转染至SW620细胞,...
However, miR-23b-3p mimics and knockdown of α-synuclein reversed the effect of MALAT1 on autophagy and the inflammatory response of BV-2 cells. In addition, after coculture of BV-2 cells with α-synuclein, the level of autophagy further decreased when 3-MA was added...