The miR-223-3p inhibitor exhibited the opposite effect, causing hyperactivation of NLRP3 inflammation activation and downstream pathways. Additionally, we further demonstrated that miRNA-223-3p inhibited the secretion of IL-1尾 and IL-18 by regulating the NLRP3/caspase-1 pathway. Furthermore, ...
miR-223-3p inhibitor Sense: 5 ′-UGGGGUAUUUGACAAACUGACA-3 ′ miRNA inhibitor control Antisense: 5 ′-CAGUACUUUUGUGUAGUACAA-3 ′ 2.4. RNA Interference For RNAi transfection, 2 × 106 cells/well were seeded onto a 6-well plate. siRNA duplexes were transfected into BMDCs...
过继转移miR-223-3p inhibitor转染的Th17细胞的小鼠EAU明显轻于接受对照inhibitor转染的Th17细胞的小鼠,主要表现为临床评分降低及眼部炎性细胞浸润和视网膜结构紊乱减少;接受miR-223-3p inhibitor转染的Th17细胞的小鼠T细胞中IL-17,IFN-γ的产生量和Th17,Th1细胞的百分比均明显低于对照组;接受miR-223-3p inhibitor转染的...
Co-transfection of AMO-223-3p (an antisense inhibitor of miR-223-3p) reversed the repressive effect of miR-223-3p. Luciferase assay showed that miR-223-3p, but not the negative control, substantially suppressed the luciferase activity, confirming the direct binding of miR-223-3p to the seed...
淫羊藿苷(质量分数>98%,批号171123)购自南京泽郎生物科技有限公司;miR-223-3p拮抗剂(miR-223-3p antagomir)及其阴性对照(NC)由广州锐博生物科技有限公司合成;miR-223-3p拟似物(miR-223-3p mimic)、miR-223-3p抑制剂(miR-223-3p inhibitor)及其NC由上海吉玛制药技术有限公司合成;TUNEL试剂盒(批号GS008)购自上...
采用细胞转染技术改变细胞中miR-223-3p的内源性表达水平,将其分为:①空白对照组;②NC组;③miR-223-3p mimic 组;④miR-223-3p inhibitor 组.成骨诱导分化 7天后,检测成骨标志性基因Runx2和OPN的表达,成骨诱导21天后,茜素红染色观察钙化结节表达.4.miR-223-3p靶基因的验证:生物信息学分析和靶基因预测软件...
Compared with the NC group, the expression of miR-223-3p and the mRNA and protein expression of Bax were significantly decreased in the miR-223-3p inhibitor group, while the mRNA and protein expression of ECT2, Bcl-2, VEGF and TGF-尾1 were significantly increased (both P<0...
目的探究模拟失重下微血管内皮细胞通过外泌体转运miR-223-3p对成骨细胞分化的影响,为失重性骨质丢失的防治寻求干预靶点.方法利用2D回转器对小鼠微血管内皮细胞进行模拟失重处理,采用低温超高速离心法提取细胞培养上清中的外泌体,对外泌体进行鉴定及摄取实验;向MC3T3-E1细胞中转染mimic-223-3p,inhibitor-223-3p及相应...
In addition,LARP4knockdown increased the proliferation, migration, and invasion of EOC cells inhibited by miR-223-3p inhibitor. Mechanically, circBNC2 regulatesLARP4expression in EOC cells by spongy miR-223-3p. In addition, in-vivo studies have shown that overexpression of circBNC2 inhibits tumor...
分别将miR-223-3p mimics,miR-223-3p inhibitor和siRAC1转染至SMMC-7721细胞,通过CCK-8,克隆形成实验和Annexin V-FITC/PI染色流式细胞术检测SMMC-7721细胞的增殖,克隆形成和凋亡水平.用双荧光素酶报告基因实验检测miR-223-3p与RAC1的靶向关系,Western blotting检测细胞中RAC1蛋白的表达水平.结果:miR-223-3p在HCC...