通过体外转染,评估CRISPR-Cas9系统对mmu-miR-21a的编辑效果,包括mRNA水平的变化和细胞增殖率的影响。对转染后的细胞进行GFP阳性和阴性分选,进一步分析3xFLAG标签的表达和mmu-miR-21a-5p的表达水平,结果显示与对照组相比,转染单质粒的CT-2A细胞中mmu-miR-21a水平显著降低,细胞增殖率降低。随着质粒剂量的增加,转染效率...
3月25日,中山大学中山眼科中心卓业鸿/苏文如教授团队在国际著名期刊Biomaterials杂志上发表了题为“TNF-α stimulation enhances theneuroprotective effects of gingival MSCs derived exosomes in retinalischemia-reperfusion injury via the ...
The above results indicate that miR‐21a negatively modulates two tumor suppressor genes, miR‐200c and PTEN, thereby promoting M2 macrophage transformation. This demonstrates that miR‐21a represents a novel target for improving the overall tumor microenvironment.Ning Li...
肾脏功能;超声心动检测小鼠心脏功能,评估维格列汀对小鼠糖尿病心肌病变的改善作用及安全性;观察小鼠心脏组织形态学变化;应用免疫组化以及westernblot检测心脏电生理因子(connexin 43),自噬功能(LC3,P62)及相关调节因子蛋白(PTEN,Akt,mTOR)的表达变化;应用qRT-PCR法检测维格列汀对各组小鼠心脏组织miR-21a-5p水平的影响....
目的:粪肠球菌导致的脑膜炎已引起了较大的公共卫生安全问题,但关于其穿越血脑屏障的机制尚不清楚,本研究从宿主-病原菌相互作用的角度入手,利用smallRNA测序技术,结合多种生物信息学分析手段,筛选,挖掘与血脑屏障通透性改变相关的调节基因和关键通路,以期为揭示粪肠球菌脑膜炎的发生机制研究提供可靠的理论依据. 方法...
(2)KEGG途径分析发现,两组中均发现了KEGG相关的富集途径,如氧化磷酸化途径,其与帕金森病,亨廷顿病,阿尔茨海默病和心肌收缩相关;(3)实验还发现miR-21a-5p可能是肌萎缩侧索硬化途径中的潜在生物标志物,因为miR-21a-5p在该途径中表达下调;(4)上述数据说明,实验成功检测出了在脊髓损伤中发挥潜在作用的一些重要蛋白...
Micro RNA-21a (miR-21a) was found to function as a negative regulator of Nur77. Moreover, we showed that miR-21a plays an inhibitory role in anaphylaxis. Altogether, the findings of our study suggest that the Nur77-miR-21a loop can function as a target for developing anti-allergy drugs...
6, No. 11 miR-211 suppresses hepatocellular carcinoma by downregulating SATB2 Guixing Jiang1, Yunfu Cui2, Xin Yu3, Zhengrong Wu1, Guoping Ding1, Liping Cao1 1Department of Hepatopancreatobiliary Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University...
3.4功能性外泌体中miR-1249-5p基因表达 如图3所示,与对照组比较,模型组外泌体miR-1249-5pmRNA表达水平显著下降(P<0.01);与模型组比较,各给药组外泌体miR-1249-5pmRNA表达水平明显增加(P<0.05、0.001),而含药血清低剂量组miR-...
TNF-α stimulation enhances the neuroprotective effects of gingival MSCs derived exosomes in retinal ischemia-reperfusion injury via the MEG3/miR-21a-5p axis 来自 dx.doi.org 喜欢 0 阅读量: 77 作者:Z Yu,Y Wen,N Jiang,Z Li,J Guan,Y Zhang,C Deng,L Zhao,SG Zheng,Y Zhu ...