进一步地,所述的miR-219a-5p的有效剂量为6～20μl/kg。进一步地,所述的药物是注射药剂。与现有技术相比,本发明的优点在于通过大量的实验证实,在建立甲基苯丙胺大鼠模型中,甲基苯丙胺引起microRNA在伏隔核表达发生变化,靶向AT1的miR-219a-5p伏隔核过表达后,对条件性线索诱导的大鼠甲基苯丙胺复吸行为以及甲基苯...
摘要 目的探讨血清miR-219a-5p在孤立性眩晕患者中的表达水平及临床意义。方法将我院2021年4月至2022年8月住院治疗的66例孤立性眩晕患者设为研究组,另选取同期66例健康体检者为对照组;采用qRT-PCR法检测血清...展开更多 Objective To explore the expression level and clinical significance of serum miR-219a-5p...
目的 探究miR-219a-5p对人心肌细胞(HCM)氧化应激损伤的作用和机制.方法 利用H2O2建立HCM氧化应激模型,将HCM细胞随机分成4组:对照组,H2O2组(200μmol/L H2O2处理6 h),H2O2+NC mimic组(H2O2处理前转染对照mimic)和H2O2+miR-219a-5p组(H2O2处理前转染miR-219a-5p mimic).噻唑蓝(M T T)法检测细胞活力,流式...
从这2组中预测miR-219-5p的潜在靶点.采用MTT法,流式细胞术和Transwell实验初步验证miR-219-5p对GBM细胞增殖,凋亡和侵袭的影响.结果:60例GBM组织中筛出14个与增殖和凋亡相关的基因和5个与侵袭相关的基因(P0.001);在这19个候选基因中预测到4个基因(TWIST1,MYO1B,WEE1和SPRED2)是miR-219-5p的潜在靶点.实验...
miR-181a-5p下拉实验也显示CircHMGB2显著富集(Fig. 3E)。此外,qRT-PCR结果显示,在H1299细胞中,敲除CircHMGB2表达后,miR-181a-5p水平升高(Fig. 3F)。在H1299细胞中,FISH染色显示CircHMGB2和miR-181a-5p在细胞质中共定位(Fig. 3G)。预测CircHMGB2可能通过海绵吸附miR-181a-5p来发挥其生物学功能,CARM1为...
Figs 5a/c, "The apoptotic rate of Ishikawa cells is significantly enhanced after miR-29a-5p mimic and siTPX2 transfection compared to NC cells, as assessed by FCM ... The apoptotic rate of KLE cells is significantly enhanced after miR-29a-5p mimic and siTPX2 transfection compared to NC ...
Tan X, Fu Y, Chen L, et al. miR-671-5p inhibits epithelial-to-mesenchymal transition by downregulating FOXM1 expression in breast cancer.Oncotarget. 2016;7:293–307. doi:10.18632/oncotarget.634426588055 PubMedGoogle Scholar Della Vittoria Scarpati G, Falcetta F, Carlomagno C, et al. A spec...
(C) Colony-formation assay showed the effect of miR-671-5p on the proliferation of SW620 and LOVO cells. *P<0.05, **P<0.01, ***P<0.001. Display full size Figure 4 miR-671-5p promoted migration and invasion of colon cancer cell (SW620 and LOVO) in vitro. (A) Transwell migration ...