总结 总的来说,我们证明了miR-218-5p过度表达的外泌体加速了生长期的发生,而球状培养提供了细胞治疗的潜在途径。miR-218-5p通过下调WNT信号抑制剂SFRP2来调节毛囊发育,从而促进β-连环蛋白形成正反馈回路。与目前的头发再生实践相比...
ANKRD1细胞凋亡炎症目的:探讨miR-218-5p对地塞米松诱导的人成骨细胞损伤的影响和可能机制.方法:体外培养人成骨细胞HFOB1.19,分别转染miR-218-5p模拟物,ANKRD1小干扰RNA或共转染miR-218-5p模拟物与ANKRD1过表达载体,然后采用10μmol/L地塞米松干预转染后的细胞24 h,RT-qPCR检测细胞中miR-218-5p和ANKRD1 mRNA表达...
以白血病细胞K562为研究对象，分别构建过表达miR -218-5p 或沉默BCL3的K562细胞株，噻唑蓝（MTT ）法检测细胞增殖，流式细胞仪检测细胞凋亡，Western blotting 检测细胞增殖抗原（Ki67）、细胞周期蛋白D1（Cyclin D1）和活化的半胱氨酸天冬氨酸蛋白酶（Cleaved Caspase -3）的表达水平。双荧光素酶报告基因实验和...
Moreover, SOCS3 was highly expressed and miR-218-5p was downregulated in the OVX rat models, and silencing SOCS3 or overexpressing miR-218-5p alleviated POP in OVX rats by promoting osteogenesis. Conclusion SOCS3 downregulation mediated by miR-218-5p increases osteoblast differentiation to ...
MiR-218-5p upregulation promoted the BMSC osteogenic differentiation, while SOCS3 overexpression reversed the effects of miR-218-5p. Moreover, SOCS3 was highly expressed and miR-218-5p was downregulated in the OVX rat models, and silencing SOCS3 or overexpressing miR-218-5p alleviated POP in ...
(F) Transfection of mimics or NC targeting miR-218-5p and measurement of miR-218-5p expression levels in cells by qRT‒PCR. (G, H) A dual-luciferase reporter gene assay was used to measure luciferase activity in SK-MEL-28 cells Full size image We mutated the binding sequence of miR-...
应用qRT-PCR,Western印迹,免疫组化技术检测内髁,外髁中miR-218-5p和SOST的表达情况,对数据进行分析.结果:miR-218-5p和SOST在内外髁的软骨下骨中均有表达.通过qRT-PCR检测到内翻型膝关节炎中miR-218-5p在软骨下骨中的表达量内髁明显高于外髁(P<0.01),SOST在软骨下骨的相对表达量内髁低于外髁(P<0.01)....
3.4. MiR-218-5p Targeting the 3′UTR of ACSL1 Bioinformatics analysis revealed that hsa-miR-218-5p is a potential miRNA for targeting the human ACSL1 3′UTR (Supplementary Figure S3). Moreover, the mature sequence of miR-218-5p and its binding site for ACSL1 was highly conserved among ...
货号:ssc-miR-218-5p 原价:￥4030 现价:￥3098.38节省:￥931.62 规格:1 kits 说明:ssc-miR-218-5p 特异性茎环引物试剂盒 ￥615 产品参数 ---说明书下载--- microRNAs(miRNAs)是一类长度约19~24nt的非编码单链RNA,通过碱基互补配对的方式与靶基因的29’UTR区部分或完全互补,剪切靶基因的转录产物或者抑制转录...
目的:本课题主在探讨过敏性紫癜(HSP),紫癜性肾炎(HSPN)患儿外周血miR-218-5p的表达与高迁移率族蛋白1(HMGB1)表达水平之间的相关性,并与临床实验室指标进行比较,分析相关性,探究miR-218-5p,HMGB1在HSP,HSPN患儿发病机制中发挥的生物学作用. 方法:选取2019年11月至2020年8月在内蒙古自治区人民医院儿科住院治疗.....