近日,来自河北医科大学心血管医学中心的研究者们在J Adv Res杂志上发表了题为“Hsa_circ_0001402 alleviates vascular neointimal hyperplasia through a miR-183-5p-dependent regulation of vascular smooth muscle cell proliferation, migration, and autophagy”的文章,该研究表明Hsa_circ_0001402作为miR-183-5p海绵,...
葡萄糖转运蛋白乳酸脱氢酶增殖目的探讨miR-183-5p/mTOR信号轴是否通过影响胃癌细胞有氧糖酵解过程而促进胃癌细胞增殖,为胃癌的治疗提供新靶点.方法使用转染试剂盒对MGC-803胃癌细胞进行inhibitor NC,miR-183-5p inhibitor,mimics NC,miR-183-5p mimics转染;葡萄糖,乳酸,腺嘌呤核苷三磷酸(adenosine triphosphate,ATP)...
目的 探讨破骨细胞来源外泌体中miR-183-5p对肺腺癌细胞侵袭和增殖的影响及其可能的机制。 方法 采用RAW264.7细胞诱导分化破骨细胞并提取外泌体,通过透射电子显微镜及激光粒度仪进行鉴定。将外泌体与肺腺癌细胞A549细胞共培养,采用qRT-PCR检测A549细胞中miR-183-5p表达水平。采用划痕实验和Transwell侵袭实验检测miR-...
Results FHL1 expression was downregulated in BC tissues and cells, whereas miR-183-5p and miR-96-5p were upregulated in BC tissues (negative correlation with FHL1 expression). FHL1 overexpression inhibited the viability, colony number, migration, and invasion of BC cells and the expression of ...
抑制细胞增殖并促进细胞凋亡.荧光素酶报告基因实验确认FAT1是miR-183-5p靶向基因.GC肿瘤组织miR-183-5p和FAT1水平呈现出明显负相关.将miR-183-5p转染入GC细胞BGC-823内能够抑制其在移植瘤模型中的生长.结论miR-183-5p潜在靶标是FAT1,miR-183-5p通过抑制FAT1负性调控GC的发展,miR-183-5p有望成为GC潜在的诊断...
Luciferase assays confirmed the binding of miR-183-5p and TREK-1. In addition, over-expression of TREK-1 blocked the roles of miR-183-5p in neuropathic pain. Our findings suggested that miR-183-5P participated in the regulation of CCI-induced neuropathic pain through inhibiting the expression ...
摘要 目的探讨破骨细胞来源外泌体中miR-183-5p对肺腺癌细胞侵袭和增殖的影响及其可能的机制。方法采用RAW264.7细胞诱导分化破骨细胞并提取外泌体,通过透射电子显微镜及激光粒度仪进行鉴定。将外泌体与肺腺癌细胞A549细...展开更多 Objective To explore the effect of osteoclast-derived exosome miR-183-5p on the...
货号:ola-miR-183-5p 原价:￥4030 现价:￥3098.38节省:￥931.62 规格:1 kits 说明:ola-miR-183-5p 特异性茎环引物试剂盒 ￥615 产品参数 ---说明书下载--- microRNAs(miRNAs)是一类长度约19~24nt的非编码单链RNA,通过碱基互补配对的方式与靶基因的29’UTR区部分或完全互补,剪切靶基因的转录产物或者抑制转录...
目的探讨miR-183-5p对大肠癌(CRC)细胞增殖,迁移能力的影响及机制.方法应用miRDB数据库,根据重组人抑制蛋白-2(PFN2)基因序列预测筛选的miR-183-5p;应用实时荧光定量PCR(qRT-PCR)法检测CRC组织及细胞系中miR-183-5p和PFN2 mRNA表达并分析其相关性;荧光素酶报告基因技术检测miR-183-5p与PFN2 3'非翻译区(3'UTR...
MicroRNAs (miRNAs) are regarded as regulators of tumorigenesis and it is known that miR-183-5p is significantly upregulated in non-small cell lung cancer (NSCLC), suggesting it has an oncogenic function in lung cancer. In this study, we found that miR-183-5p could promote lung carcinogenesis...