结果显示miR-181a和miR-181d-5p在脂肪组织中高表达.经miRandn和Target Scan miRNA的靶基因预测软件预测获得miR-181a和miR-181d-5p的靶基因分别为GPD2和CREB1,以成华猪背部皮下脂肪组织和前脂肪细胞为研究对象,通过实时荧光定量PCR,双荧光素酶报告实验和细胞转染实验等方法,探究miR-181a和miR-181d-5p对猪脂肪...
Among these, only miR-181d-5p significantly affected RUNX2 gene expression based on overexpression and knockdown experiments. From the osteogenesis-related gene and protein expression, as well as alkaline phosphatase and alizarin red experiments, we further confirmed that the downregulation of miR-181d...
However, the function of miR-181d-5p in regulating the BTB has not been directly documented. SOX5 (sex determining region Y-box protein 5), located in paralogous chromosomal regions on p12.1, is a member of the SOX family of transcription factors that play master roles in the regulation ...
Objective To investigate effects of aconitine on proliferation and apoptosis of cervical cancer HeLa cells by regulating the microRNA-181d-5p (miR-181d-5p)/DEAD-box RNA helicase 3 (DDX3) axis. Methods Cervical cancer HeLa cells were treated with different doses of aconitine, and...
微小RNA-181d-5pDEAD盒多肽3放射敏感性目的探讨微小RNA-181d-5p(miR-181d-5p)对宫颈癌细胞放射敏感性的影响及机制.方法X射线照射构建放疗抵抗的宫颈癌细胞株HeLa-X,qRT-PCR法检测人宫颈癌细胞SiHa,HeLa,C33A,HCC-94,HeLa,HeLa-X及人正常宫颈上皮细胞HCerEpic中miR-181d-5p表达,使用Lipofectamine^(TM)2000...
miR-181d-5p is therefore a new therapeutic target for the development of anti-hypercholesterolemia drugs.Yu WangFan LiXiaoqian GaoHuahui YuZhiyong DuLinyi LiYunhui DuChaowei HuYanwen QinJournal of Endocrinology
miR-181amiR-181d-5p脂肪细胞【目的】探究miR-181a和miR-181d-5p在荣昌猪原代前体脂肪细胞分化中的作用及机制.【方法】通过比对种子序列差异分析miR-181a和miR-181d-5p在猪,人,大鼠和小鼠不同物种间的序列保守性;实时荧光定量PCR检测miR-181a和miR-181d-5p在猪肌肉和背部皮下脂肪组织中表达;利用miRandn和...
miR-181d-5pCDKN3human umbilical vein endothelial cellsEndothelial cell (EC) dysfunction induces atherosclerotic coronary heart disease (CHD) development. Recent studies demonstrated that lncRNA NEAT1 mediates multiple biological functions of cells. How NEAT1 regulates EC function is still unclear, so ...
miR-181d-5p对宫颈癌细胞放射敏感性的 影响及机制doi:10.3969/j.issn.1002-266X.2022.22.010李祥双于海霞叶燕珍温慧敏杜丽苹傅丽玲于卫华Shandong Medical Journal
Mechanistically, exosomal miR-181d-5p transferred from CAFs to RCC cells directly suppressed the expression of ring finger protein 43 (RNF43) and activated Wnt/尾-catenin signaling pathway, thus promoted cancer stemness and tumor progression. Overexpression of RNF43 strongly suppressed stemness ...