目的:探究miR-141-3p通过调控胰岛素样生长因子1/血小板源性生长因子对腰椎间盘突出症大鼠背根神经节炎症及下肢疼痛的影响。 方法:选取50只SPF级SD雄性大鼠,随机分为正常组、模型组、miR-NC组、miR-141-3p inhibitor组、miR-141-3p...
Inhibition of miR-141-3p significantly enhanced the expression of Keap1, the target gene of miR-141-3p, and thus downregulated Nrf2, while activation of Nrf2 reversed the reduction in M2 polarization by miR-141-3p inhibitor. Collectively, miR-141-3p contributes to tumor progression, migration, ...
-141-3p in the cytoplasm (Fig.5E). A lower miR-141-3p level can be found in CRC tissues compared to normal tissues, as revealed by GSE41655 and GSE48267 datasets (Fig.S3B, C). Additionally, miR-141-3p mimics could downregulate HIF1A-AS2 expression, whereas miR-141-3p inhibitor ...
A. qRT-PCR analysis of miR-141–3p levels to validate the efficiency of the miR-141–3p mimics and inhibitor in both HCT116 and SW480 cells. B. The effects of miR-141–3p mimics and inhibitor on HCT116 and SW480 cells proliferation as determined by the MTT assay. C and D. ...
To validate that the antitumor activity of AIF in TPC-1 and BCPAP cells was mediated by upregulating miR-141-3p, we downregulated the expression of miR-141-3p through the transfection with miR-141-3p inhibitor (Fig. 3C). We next examined whether the downregulation of miR-141-3p affects the...
The miR-141-3p has been reported to participate in regulating autophagy and tumor-stroma interactions in ovarian cancer (OC). We aim to investigate whether miR-141-3p accelerates the progression of OC and its effect on macrophage 2 polarization by target
The rejuvenating effects of PDLSC-Exos were notably reversed by cotreatment with ML385, an inhibitor of nuclear factor erythroid 2-related factor 2 (NRF2), indicating that this recovery depended on NRF2 activation. Further analyses revealed that microRNA-141-3p (miR-141-3p) was expressed at ...
④背根神经节组织胰岛素样生长因子1、血小板源性生长因子蛋白表达:模型组明显低于正常组(P < 0.05),miR-141-3p inhibitor组明显低于miR-NC组(P < 0.05),miR-141-3p mimics组明显高于miR-141-3p inhibitor组(P < 0.05)。⑤胰岛素样生长因子1与miR-141-3p呈正相关(r=0.904,P < 0.001),血小板源性生长因子...
选择人正常肝细胞株HL-7702,并选择人肝癌细胞株Hep-3B和Huh7,构建转染pc-DNA3.1-SNHG16的pc-DNA3.1-SNHG16组,转染pc-DNA3.1-SNHG16-siRNA的si-SNHG16组,转染miR-141-3p inhibitor的miR-141-3p inhibitor组,转染miR-141-3p mimic的miR-141-3p mimic组,转染pc-DNA3.1-FOXJ3-siRNA的si-FOXJ3组.应用...
目的研究miR-141-3p介导Yes相关蛋白1(YAP1)基因表达对晚期非小细胞肺癌(NSCLC)细胞侵袭转移和化疗敏感性的影响及其作用机制.方法收集50例晚期NSCLC及癌旁组织标本,采用定量逆转录聚合酶链反应(qRT-PCR)检测组织中miR-141-3p和YAP1表达量.选择NSCLC细胞株,设计miR-141-3p inhibitor和siRNA-YAP1,将细胞分为Blank组...