Mir-X miRNA qRT-PCR SYBR Kits are complete, dual-function systems for performing first-strand cDNA synthesis and quantitative PCR (qPCR) to precisely measure the level of your favorite miRNAs. The kits are available in economical, large-sized formats that provide 200 or 600 qPCR reactions, and...
•Mir-X miRNA First-Strand Synthesis and TB Green qRT-PCR Kit User Manual Quality Control Data cDNA was synthesized using serial dilutions of a synthetic miRNA standard and the components of the kit. The samples were then subjected to miRNA analysis using the TB Green Advantage qPCR Premix. ...
产品英文名称:Mir-X™ miRNA First Strand Synthesis Kit 货号:pro_27591产地:clontech 规格/价格:询价 产品详细说明: Mir-X™ miRNA qRT-PCR SYBR®Kitsare complete, dual-function systems for performing first-strand cDNA synthesis and quantitative PCR (qPCR) to precisely measure the level of your ...
¥2847.00 价格:2847.00 购买此商品赠送2847积分 自营Takara- Mir-X miRNA First-Strand Synthesis Kit/20次... 折扣:10 销量:17 TAKARA宝日医生物 优惠: 惠 购买此商品赠送: 2847积分 已选:20次/盒;数量:1 送至: 运费:
Clontech 638315 Mir-X miRNA First-Strand Synthesis Kit 60 Rxns ￥4,820 ￥3,615 Clontech 638313 Mir-X miRNA First-Strand Synthesis Kit 20 Rxns ￥3,127 买一赠一 Clontech 638314 Mir-X miRNA qRT-PCR TB Green® Kit 20 Rxns RT;200 Rxns qPCR ￥4,867 ￥3,650 Clontech 638316 Mir-X...
用mir-xmirnafirst-strandsynthesiskit(clontech,mountainview,ca)反转录试剂盒对mirnas进行逆转录,使zma-mir164e通过逆转录后携带标签序列能被定量。用zma-mir164e的整个序列作为5’引物,试剂盒提供的标记序列的一部分作为3’引物,用半定量rt-pcr方法对转基因拟南芥中zma-mir164e的表达量进行定量分析。
总RNA的提取采用Qiagen试剂盒,mRNA的反转录采用PrimeScriptTMRT Master Mix,miRNA的反转录采用Mir-X miRNA First-Strand Synthesis,PCR按SYBR RTFQ-PCR Kit试剂盒说明书操作,mRNA检测以GAPDH为内参,miRNA检测以U6作为内参,使用7900HT...
以U6为内参,RNA提取试剂盒提取总RNA,Mir-X miRNA First-Strand Synthesis kit逆转录为cDNA,SYBR Premix Ex Taq定量PCR试剂盒检测miR-552的表达,采用2-△△Ct法计算miR-552的相对表达量。miR-552上游引物序列(5′–3′):CAGGTGACTGGTTAGAC;下游引物序列(5′–3′):GAACATGTCTGCGTATCTC。U6上游引物序列(5...
2.5 差异表达 miRNA 的 qRT-PCR 验证 选择 miR-199b-5p 这个差异表达 miRNA 进行 qRT-PCR 验证 . 取各组大鼠 miRNA , 使用 TransScriptⅡAll-in-One First-Strand cDNA Synthesis SuperMix for qPCR(One-Step gDNA Removal)反转 录试剂盒反转录成 cDNA.根据 TransStart Top Green qPCR SuperMix 试剂盒说明...
U6 was used as the internal control (The U6 primers were provide by Mir-X™ miRNA Frist-Strand Synthesis Kit). The relative gene expression was calculated with the 2−ΔΔCT methods. Data were shown as mean ± SD from three independent experiments in triplicates. The primer ...