核转录因子CDX2基因在非小细胞肺癌(NSCLC)发病机制中的作用尚不清楚。在本研究中,我们通过qRT-PCR发现CDX2 可上调miR-744的表达。染色质免疫沉淀实验证实CDX2直接与miR-744的启动子区域结合。荧光素酶报告基因检测进一步验证了CDX2Y最可...
通过生物信息学预测miR-744-5p的下游靶分子,qRT-PCR和Western blot验证CCND1在786-O及OSRC2细胞中的表达水平,双荧光素酶报告基因实验进一步验证miR-744-5p和CCND1的关系,最后通过回复实验验证miR-744-5p/CCND1轴在ccRCC中的作用。结果 MiR-744-5p在ccRCC组织和细胞系中显著下调(P<0.05),过表达miR-744-5p可以...
结果发现非小细胞肺癌患者血清外泌体miR-744-5p的表达水平(0.012 2±0.019 7)较健康对照组(0.093 6±0.081 9)明显下调,差异有统计学意义(U=553.5,P<0.001)。在肺癌组中,早、晚期肺癌患者血清外泌体miR-744-5p的表达水平(0.011 7±0.013 9,0.012 6±0.021 2)与健康对照组相比,均明显降低(U=72.5,U=471.5...
MCE 国际站:hsa-miR-744-3p agomir 产品活性:hsa-miR-744-3p agomir 是一种经过特殊化学修饰的miRNA模拟物,miRNA成熟链全链进行甲氧基修饰,在5'端和3'端分别有2个和4个硫代骨架修饰,并在3'端连接有高亲和性胆固醇修饰。miRNA agomir 可以模拟内源性miRNA,上调miRNA活性。与普通模拟物相比,miRNA agomir在动物...
MCE 国际站:hsa-miR-744-5p antagomir 产品活性:hsa-miR-744-5p antagomir 是一种经过特殊化学修饰的成熟miRNA的互补单链,全链进行甲氧基修饰,在5'端和3'端分别有2个和4个硫代骨架修饰,并在3'端连接有高亲和性胆固醇修饰。miRNA antagomir通过特异性结合成熟miRNA,阻止miRNA与其靶基因的互补配对,从而抑制miRNA...
miR-744-5p silencing promoted MM cells prolifera tion, invasion, migration, glucose uptake, lactate production, and EMT by elevating SOX12 (P<0.01). miR-744-5p inhibited the growth of MM xenograft tumors in vivo (P<0.001). Conclusion: miR-744-5p inhibits MM cells proliferation, invasion...
０．０５).L I N C ０１１０６靶向负调控m i R Ｇ７４４Ｇ５p ,干扰m i R Ｇ７４４Ｇ５p 可逆转干扰L I N C ０１１０６对M D A ＧM B Ｇ２３１细胞增殖㊁迁移和侵袭的抑制作用.结论㊀L I N C ０１１０６在乳腺癌组织中表达升高,其可能通过靶向负调控m i R Ｇ７４４Ｇ５p 促进乳腺癌细胞...
www.nature.com/scientificreports OPEN received: 14 January 2015 accepted: 05 May 2015 Published: 11 August 2015 miR-744 enhances type I interferon signaling pathway by targeting PTP1B in primary human renal mesangial cells Xiaoyan Zhang1,*, Xiao Han2,*, Yuanjia Tang1, Yanfang Wu1,...
We found that the activation of TYK2, STAT1 and STAT3 was significantly enhanced by miR-744. miR-744 also enhanced the activation of non-classical signal components, such as ERK and p38. We then identified PTP1B, a ubiquitously expressed phosphatase, as the target of miR-744 that is ...