双荧光素酶报告基因实验进一步表明,与转染突变体mir -30a-5p的细胞相比,转染wt-miR-30a-5p的细胞荧光素酶活性降低。这一结果表明,snail是miR-30-5p的靶点,而circMET可以通过miR-30-5p/ snail促进HCC的进展。考虑到snail作为一种转...
科学家发现CLCF1通过激活PI3K/AKT信号通路及其下游基因来促进糖酵解,从而参与索拉非尼耐药肝癌细胞的糖酵解。此外,还发现miR-30a-5p直接靶向CLCF1,索拉非尼介导的miR-30a-5p抑制索拉非尼耐药的HCC细胞中CLCF1的上调。此外,当胆固醇修饰的agomiR-30a-5p系统传递给索拉非尼耐药肝癌小鼠时,肿瘤生长显著下降。在HCC中,...
此外,当胆固醇修饰的agomiR-30a-5p系统传递给索拉非尼耐药肝癌小鼠时,肿瘤生长显著下降。在HCC中,miR-30a-5p/ CLCF1轴介导索拉非尼耐药和有氧糖酵解激素治疗的生化耐药机制尚不明确。因此,本研究表明,靶向miR-30a-5p/CLCF1轴有望对肝癌索拉非尼耐药患者实现治疗干预。 RIPA (Abmole, M9637,纯度>99%) 是一种...
Figure 1. miR-30a interactions with BNIP3L. (A) Schematic of the BNIP3L 3’UTR region showing the putative miR-30a binding sites. (B) Relative mRNA expression of BNIP3L in keratinocytes 24 h after transfection of miR-30a-3p or miR-30a-5p mimics or a scrambled sequence (NC: Negativ...
英文名称: hsa-mir-30a-5p Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit 品牌: Abbexa 货号: abx097646 规格: 50 rxns 价格: $493 产品说明书下载: 产品预定 >> 产品预定,提交订单信息之后,我们会第一时间与您取得联系 试剂和工具 试剂...
专利摘要:本申请涉及生物医药领域,公开了一种外泌体hsa‑miR‑30a‑5p在制备脓毒症血管损伤诊断试剂盒中的应用,其核苷酸序列如SEQIDNO:1所示。本申请首次揭示脓毒症诱导的内皮细胞及其分泌的外泌体参与临近血管内皮细胞损伤,放大全身血管损伤,引起全身多器官损伤。发现脓毒症时血管内皮细胞源性外泌体中hsa‑...
Interestingly, miR-30a-5p was observed to inhibit the nonamyloidogenic pathway by down regulating ADAM10 and SIRT1, thus promoting Aβ1-42 overproduction. In APP/PS1 mice, knockdown of miR-30a-5p ameliorated cognitive dysfunctions and neurodegenerative changes, suppressed Aβ accumulation, and ...
目的:探讨miR-30a-5p靶向三结构域蛋白31(TRIM31)基因对结直肠癌(CRC)细胞5-氟尿嘧啶(5-FU)耐药的影响,并阐明其作用机制.方法:收集27例5-FU化疗敏感(5-FU/S组)CRC患者和23例5-FU化疗耐药(5-FU/R组)CRC癌患者的CRC组织,实时荧光定量PCR(RT-qPCR)法检测癌组织中miR-30a-5p和TRIM31 mRNA表达水平,Pearson...
chi-miR-30a-5p qPCR试剂盒 分类:microRNA qPCR Kits 品牌:BioTNT 货号:chi-miR-30a-5p 原价:￥4030 现价:￥3098.38 节省:￥932 规格:1 kits 说明:chi-miR-30a-5p 特异性茎环引物试剂盒 ￥615产品参数 ---说明书下载--- microRNAs(miRNAs)是一类长度约19~24nt的非编码单链RNA,通过碱基互补配对的方...
Functional studies showed that the miR-30a-5p/CLCF1 axis plays an important role in regulating both sorafenib resistance and aerobic glycolysis in sorafenib-resistant cells. Taken together, our findings reveal that the miR-30a-5p/CLCF1/PI3K/AKT regulatory cascade is part of an important mechanism...