2024年底,AI玩具的潜力已经展露。卡西欧联合初创公司Vanguard Industries推出的AI毛绒宠物玩具Moflin,单价折合人民币约3000元,却在预售期直接售罄;特斯拉推出售价40美元的1:10迷你版人形机器人玩具,也在上线24小时内售罄。 时间来到2025年,1月7日至10日,CES(国际消费电子产品展览会)在美国拉斯维加斯举办,作为世界上最...
编码器 PBMN-24B2HRA11409211000/S01BAUMER 温度传感器 TED50 G1/2 0+10 BAR R M12 8B 5M BLBAUMER 编码器 MHAP200 B5 G180 SG13N P1024 DBAUMER 编码器 OM70-P0140.HH0100.EKBAUMER 编码器 MIR3000F-0395.MT.032768.ABAUMER 电感式传感器 IFRM 04N15A3/S05LBAUMER 编码器 EIL580P-SC10.5RQ....
3A). The results showed that miR-141 could significantly cause 50-60% reduction in the luciferase signals in the wild-type (pmir-YAP1-WT) but not in the mutant (pmir-YAP1-MT) of YAP1 3’UTR (Fig. 3A). Western blot analysis showed that transient transfection of miR-141 caused a ...
Olympus Fluoview 3000 Laser Scanning Confocal Microscope [54]. All final figures were prepared using Adobe Photoshop software. The pupal retina was opened in Image J and ROI of 500px × 500px was drawn and the number of photoreceptor cells and the number of pigment cells were counted within...
对于与全长tlr的co-ip,接种5×106个hek293t细胞,并用大约5μg过表达带flag标签的htlr7和/或tlr8/9的质粒或过表达带ha标签的mt7/8/9的质粒进行转染。转染后24小时,如前所述,收获细胞并在冰上的300μl的多核糖体裂解缓冲液中通过超声进行裂解(keenejd等人,2006)。裂解液以14,000×g离心15分钟。100μl得...
When the cell fusion degree reached about 60%-70%, Lipo- fectamine 3000 transfection reagent was used to trans- fect mtr-miR156a mimic and NC mimic respectively. After 48 h of culture in the incubator, 10μL of CCK-8 solution(Meilunbio, Dalian, China) was added to each well. ...
E Relative expression of LINC00665 in the bound RNAs pulled down by biotinylated wild-type (wt) miR-424-5p, mutant (mt) miR-424-5p, and non-targeting control (NC) were evaluated by qRT-PCR. F the putative binding sites for BCL9L and miR-424-5p were shown. G HuCCT1-Gem and SNU...
(B) The sequence alignment of circSCP2-WT and circSCP2-MT in luciferase reporter assay for validating the binding of circSCP2 and miR-92a-1-5p. Luciferase reporter assay showing the luciferase activity of the circSCP2 luciferase reporter plasmid (WT or MUT) following transfection with miR-...
Production of mtROS in HK-2 cells was detected using MitoSOX Red Mitochondrial Superoxide Indicator (Thermo Fisher Scientific, USA). Briefly, the Mito- SOX reagent was added to Hank's balanced salt solution (HBSS) to a concentration of 5 μM. The probe solution was loaded into viable HK...
8. Harte MT, O'Brien GJ, Ryan NM, Gorski JJ, Savage KI, Crawford NT et al. BRD7, a subunit of SWI/SNF complexes, binds directly to BRCA1 and regulates BRCA1-dependent transcription. Cancer Res 2010; 70: 2538–2547. 9. Drost J, Mantovani F, Tocco F, Elkon R, Comel A, Holste...