进一步确定与circSlc8a1结合的潜在miRNAs,发现circSlc8a1和miR-20a-5p在Ago2沉淀的复合物中显著富集。Western blot分析表明,circSlc8a1敲低抑制Lamtor1表达并促进静脉SMC分化,而同时转染miR-20a-5p可逆转这些作用。此外,miR-20a-5p沉默消除了circSlc8a1对Lamtor1蛋白表达的抑制
并设立对照组(即miR-20a-5p过表达组、过表达对照组、miR-20a-5p低表达组、低表达对照组);采用生物信息学软件TargetScan预测miR-20a-5p的靶基因可能为核因子κB抑制因子β(nuclear factor kappa-B inhibitor beta,NFKBIB),分别将miR...
因此,我们提出假说:miR-20a-5p、miR-515-3p可能是通过调控PD-1抑制剂发挥作用的。方法:选取2019年1月—2021年7月于南京医科大学第四附属医院就诊的84例胃癌患者进行回顾性研究,根据治疗方案的不同将其分为观察组和对照组(每组各42例),对照组给予二线化疗方案治疗,观察组在对照组基础上给予贝伐珠单抗联合PD-1抑制...
anti-miR-20a-5p+10μmol/L HSYA+TNF-α组,CCK-8法检测细胞增殖活性,流式细胞术检测细胞凋亡,酶联免疫吸附法检测细胞培养上清液中IL-1β和IL-6表达,RT-qPCR法检测细胞中miR-20a-5p表达,蛋白质印迹法检测细胞中TLR4蛋白表达.双荧光素酶报告基因实验验证miR-20a-5p与TLR4调控关系.结果与对照组比较,TNF-α组...
“miR-20a-5p通过靶向MRTFA缓解ox-LDL诱导的内皮细胞损伤”出自《中国动脉硬化杂志》期刊2019年第9期文献,主题关键词涉及有miR-20a-5p、内皮损伤、心肌素相关转录因子A、细胞凋亡等。钛学术提供该文献下载服务。
Here we found that miR-20a-5p expression was significantly downregulated whereas its target autophagy-related gene 7 (ATG7) was increased along with clinical staging of NB progression. Correlation analysis showed that miR-20a-5p had a negative correlation trend with ATG7. In SH-SY5Y cells, forced...
货号:xtr-miR-20a-5p 原价:￥4030 现价:￥3098.38节省:￥931.62 规格:1 kits 说明:xtr-miR-20a-5p 特异性茎环引物试剂盒 ￥615 产品参数 ---说明书下载--- microRNAs(miRNAs)是一类长度约19~24nt的非编码单链RNA,通过碱基互补配对的方式与靶基因的29’UTR区部分或完全互补,剪切靶基因的转录产物或者抑制转录...
Silence of MANCR could promote cell viability, inhibit the cell apoptosis and secretion of CRP, PCT, IL-6, IL-1β, and TNF-α via miR-20a-5p / MAPK1 axis in LPS-stimulated NCI-H1563 cells (P < 0.05). High MANCR levels in pediatric SCAP patients could predict the occurrence and ...
MiR-20a-5p has been linked to growth and proliferation of breast cancer cells. Bai et al. found that miR-20a-5p overexpression promoted cell growth and proliferation both in vitro and in vivo16, while Zhao et al. found the opposite effect in vitro17. Similarly, two studies demonstrated that...