为了进一步证实miR200b-3p在SYDE1/ERK通路中的调控作用,我们将SYDE1质粒瞬时转染到miR200b-3p过表达细胞中,并将SYDE1 siRNA瞬时转染到miR200b-3p敲低细胞中并测量 P-RHOA、P62、P-ERK、LC3 和 LAMP1 的蛋白表达水平,结果提示 miR200b-3p 通过 SYDE1 影响 ERK 磷酸化来影响自噬水平(图 6F-I,图 S2G-H)...
Transcription factor ERG (erythroblast transformation-specific (ETS)-related gene) is essential in endothelial differentiation and angiogenesis, in which microRNA (miR)-200b-3p targeting site is expected by miRNA target prediction database. miR-200b is k
Histochemistry and Cell Biology (2021) 155:423–433 https://doi.org/10.1007/s00418-020-01941-2 ORIGINAL PAPER miR‑96‑5p, miR‑134‑5p, miR‑181b‑5p and miR‑200b‑3p heterogenous expression in sites of prostate cancer versus benign prostate hyperplasia—archival ...
与这些发现一致,研究者观察到在转染了miR-200c-3p的DU145 RR细胞中,DNA DSBs相关蛋白53BP1的水平比转染了对照miRNA的细胞更高(图3C,E)。综合研究者的结果表明,miR-200c-3p减少了辐射后的DNA修复,可能通过这种方式使放疗抵抗细胞重新敏感。 图3 外泌体miR-200c-3p通过阻碍DNA修复恢复放疗抵抗细胞的敏感性 4....
Our study has demonstrated that Slit2 is a target gene of miR-200b-3p and that the hypoxia-ischemic brain damage in neonatal rats was alleviated by inhibiting miR-200b-3p via Slit2. miR-200b-3p may be a potential therapeutic target of HIBD for further investigation. ...
However, the interaction between miR-200b-3p and KCNQ1OT1 or SERP1 in wound healing has not been described to date. Herein, this study focused on exploring the biological function of KCNQ1OT1 in the migration of keratinocytes, besides, whether KCNQ1OT1 performed its effects via miR-200b-...
图5 LncRNA NEAT1通过miR-124-3p/KLF4轴调控IL-13诱导的HNECs功能障碍 A.CCK-8检测细胞活力;B.流式检测细胞凋亡;C.qRT-PCR检测HNECs中lncRNA NEAT1、miR-124-3p和KLF4的表达;D.Western blot测定HNECs中KLF4、eotaxin-1 和GM-CSF...
Moreover, we found that miR-200b-3p was upregulated during monocyte/macrophage differentiation and mediated the downregulation of p38IP by binding to the 3′ untranslated terminal region of p38IP mRNA. Overexpression of a miR-200b-3p mimic resembled the effect of p38IP knockdown, whereas a ...
miR-338-3p 抑制STAT1的表达并减少炎症因子的产生[11]。然而,在LPS诱导的神经炎症模型中调控糖酵解及炎症的miRNA并不清楚。本研究以糖酵解基因PFKFB3和HK2为靶基因,结合LPS诱导的神经炎症GEO数据集,分析了其上游可能的miRNAs及其对糖酵解的...
货号:dre-miR-200b-3p 原价:￥4030 现价:￥3098.38节省:￥931.62 规格:1 kits 说明:dre-miR-200b-3p 特异性茎环引物试剂盒 ￥615 产品参数 ---说明书下载--- microRNAs(miRNAs)是一类长度约19~24nt的非编码单链RNA,通过碱基互补配对的方式与靶基因的29’UTR区部分或完全互补,剪切靶基因的转录产物或者抑制...