miR-15a and miR-16-1 in cancer: discovery, function and future perspectives. Cell death and differentiation. 2010; 17:215-220.Aqeilan RI,Calin GA,Croce CM. miR-15a and miR-16-1 in cancer: discovery, function and future perspectives.[J].Cell death and differentiation 2010,2(2)....
(外显子4和5之间)的miR-15a和miR-16-1基因簇,推测其可能参与CLL的发生,直接与BCL-2 mRNA的3'UTR序列相互作用,在转录后的水平上对BCL-2起负调节作用.本研究通过实时定量PCR(RQ-PCR)技术检测CLL患者miR-15a,miR-16-1的表达,同时对其可能的靶标Bcl-2基因进行定量检测.探讨CLL中miR-15a和miR-16-1表达与...
Here, we demonstrate that deletion in mice of the 13q14-minimal deleted region (MDR), which encodes the DLEU2/miR-15a/16-1 cluster, causes development of indolent B cell-autonomous, clonal lymphoproliferative disorders, recapitulating the spectrum of CLL-associated phenotypes observed in humans...
MiR-15aandmiR-16-1clusterfunctions inhumanleukemia GeorgeA.Calin* †‡ ,AmeliaCimmino* § ,MullerFabbri*,ManuelaFerracin ¶ ,SylwiaE.Wojcik*,MasayoshiShimizu* § , CristianTaccioli*,NicolaZanesi*,RamiroGarzon*,RamiI.Aqeilan*,HansjuergAlder*,StefanoVolinia* , LauraRassenti**,XiupingLiu*,Chan...
miR-15a and miR-16-1 act as putative tumor suppressors by targeting the oncogene BCL2. These miRNAs form a cluster at the chromosomal region 13q14, which is frequently deleted in cancer. Here, we report that the miR-15a and miR-16-1 cluster targets CCND1 (encoding cy...
miR-15a and miR-16-1(miR-15a/16-1) have been implicated as tumor suppressors in chronic lymphocytic leukemia, multiple myeloma, and acute myeloid leukemic cells. However the mechanism of inhibiting the proliferation of leukemic cells is poorly understood
miR-15a/16-1是miRNA15家族的成员,在人体细胞中是由位于染色体13q14区域的基因编码,miR-15a/16-1具有抑制细胞增殖,促进肿瘤细胞凋亡及抑制肿瘤发生等多种功能。课题组应用miRNA芯片技术筛选出肝癌组织差异表达的miRNAs,结果显示miR-15a/16-1在肝癌组织中表达显著下调,且发现临床上肝癌病人的外周血调节性B细胞的频率...
( 2008 ) The miR-15a-miR-16-1 cluster controls prostate cancer by targeting multiple oncogenic activities . Nat Med 14 : 1271 – 1277 The genes for the microRNAs miR-15a and miR-16-1 are located in the chromosomal region 13q14; deletions in this region are associated with prostate ...
MicroRNAs (miRNAs) 已被广泛报道与血液恶性肿瘤相关。在染色体13q14处丢失的miR-15a/16-1是大多数人慢性淋巴细胞白血病(CLL)的标志。小鼠miR-15a/16-1和miR-15b/16-2的缺失已被证明能促进B细胞恶性肿瘤。 实验评估了miR-15/16簇,杂交miR-15a/16-1和miR-15b/16-2基因敲除小鼠的生物学作用。出乎意料的是,...
目的:探讨mip-15a和mir-16-1诱导人淋巴瘤Raji细胞株的凋亡.方法:利用Oligofectamine2000脂质体法将化学合成的mir-15a和mir-16-1寡棱苷酸,随机序列,分别转染Raji细胞.采用间接免疫荧光及流式细胞仪检测Bel-2蛋白表达情况,半定量RT-I?CR检测Bcl-2 mRNA表达水平,台盼蓝拒染法和CCK8法检涮mir-15a和mir-16-1对...