miR-152-3p 通过靶向 FGFR3 抑制血管生成:利用 Starbase 预测并通过双荧光素酶报告基因分析、qRT-PCR 和 Western blot 验证,发现 miR-152-3p 可靶向成纤维细胞生长因子受体 3(FGFR3),抑制其表达。过表达 miR-152-3p 可抑制 HUVECs 的增殖、管腔形成和迁移,促进细胞衰老,而转染 miR-152-3p 抑制剂则产生相反...
LncRNA SNHG4 调控牙周膜干细胞成骨分化过程中的 miR-152-3p 背景:研究表明长链非编码RNA核仁小RNA宿主基因4(LncRNA SNHG4)参与了多种炎症性疾病的进展,而关于LncRNA SNHG4对牙周炎治疗过程中人牙周膜干细胞成骨分化的影响尚不明确。目的:...
然后用miR-152-3pmimics或miR-152-3p抑制剂转染U251和LN229细胞,并通过RT-qPCR评估转染效率(图5c)。U251和LN229中SLC2A1的mRNA表达和蛋白水平。通过RT-qPCR和Western blot鉴定了具有miR-152-3p模仿物或miR-152-3p抑制剂转染的细...
年6月驻马店市中心医院收治的非小细胞肺癌患者105例,收集患者入院时的临床资料,采用实时荧光定量聚合酶链反应检测非小细胞肺癌患者肿瘤组织和癌旁组织中的miR-152-3p表达水平,比较肿瘤组织和癌旁组织中miR-152-3p的表达水平,比较不同临床特征患者miR-152-3p的表达水平,采用点二列相关性分析miR-152-3p表达水平与...
说明:mdo-miR-152-3p 特异性茎环引物试剂盒 ￥615 产品参数 ---说明书下载--- microRNAs(miRNAs)是一类长度约19~24nt的非编码单链RNA,通过碱基互补配对的方式与靶基因的29’UTR区部分或完全互补,剪切靶基因的转录产物或者抑制转录产物的翻译,从而起到转录后调控靶基因的表达。 miRNAs广泛存在于动物、植物、真菌...
miR-152-3p Sensitizes Glioblastoma Cells Towards Cisplatin Via Regulation Of SOS1 OncoTargets and Therapy (2019) - 1 Comment pubmed: 31807027 doi: 10.2147/ott.s210732 issn: 1178-6930 Meihua Wang , Qi Wu , Mingming Fang , Wu Huang , Hong Zhu ...
Cancer Cell Int (2021) 21:440 https://doi.org/10.1186/s12935-021-02061-0 Cancer Cell International PRIMARY RESEARCH Open Access OIP5‑AS1 contributes to the development in endometrial carcinoma cells by targeting miR‑152‑3p to up‑regulate SLC7A5 Minglin Liang, Hongbo...
MiRNA sequencing identified miR-152-3p as a key regulator. Further investigation using dual-luciferase reporter assays, qRT-PCR, and Western blot revealed that miR-152-3p inhibits the expression of FGFR3 by binding to its 3’ UTR. Meanwhile, functional assays, including angiogenesis assays, ...
In human keloid fibroblasts and normal skin fibroblasts, miR-152-3p expression was also detected, and found that miR-152-3p expression was also up-regulated in keloid tissues and in human keloid fibroblasts. In addition, we investigated the molecular functions of miR-152-3p in keloid fibroblasts...