存活胎数及存活胎鼠重量和胎盘重量进行计量分析;④分离培养原代小鼠骨髓源性巨噬细胞(bone marrow-derived macrophages,BMDM),利用LPS刺激诱导其M1极化,再瞬时转染miR-146a-5p模拟物(miR-146a-5p mimics)或其NC片段后,通过qPCR和Western ...
将FTC-238细胞分为miR-146a-5p+wt组、NC+wt组、miR-146a-5p+mut组、NC+mut组,miR-146a-5p+wt组转染miR-146a-5p mimics与野生型(wt)质粒,NC+wt组转染阴性对照mimics-NC与野生型质粒,miR-146a-5p+mut组转染miR-146a-5p m...
经过广泛的验证，miR-96-5P被发现随着病情的发展而显著上调，而miR-146A-5P则显著下调。有趣的是，miR-96-5p和miR-146a-5p的表达水平与患者血小板水平呈强正相关。进一步的研究表明，miR-146a-5p调控了大部分参与免疫应答的蛋白的表达。这些结果表明，除了现有的生化和病理参数外，miR-96-5p和miR-146a-5p可以用...
miRNA表达谱分析和体内功能实验显示CAF中过量表达的miR-146a-5p与膀胱癌耐药的相关性。进一步结合mRNA表达谱以及功能实验,研究团队阐明了CAF中核定位的miR-146a-5p可以调控胞外基质蛋白SVEP1基因的转录,其作用机制为miR-146a-5p通过结合SVEP1基因的内含子区段,增加转录因子YY1的招募,上调SVEP1的转录水平,从而促进了CAF...
专利权项:1.miR-146a-5p过表达工程干细胞外泌体在制备治疗芥子气致肺损伤药物中的应用,其特征在于:编码miR-146a-5p的基因的核苷酸序列如SEQIDNO.1所示;所述药物为改善芥子气致肺损伤炎症反应及细胞炎症反应、改善芥子气致肺组织损伤、降低芥子气暴露肺泡灌洗液总蛋白浓度或降低芥子气致肺组织湿干重比的药物;所述...
The study of muscle disorders has gained popularity, with a particular emphasis on the relationship between adipose tissue and skeletal muscle. In our investigation, we discovered that the deletion of miR-146a-5p specifically in adipose tissue (aKO) led
3 1 6 1 2 4 6 1 1 N C CN 116421613 A 权利要求书 1/1页 1.一种过表达miR‑146a‑5p的间充质干细胞外泌体在制备治疗神经炎症的药物中的应 用。 2.根据权利要求1所述应用,其特征在于,所述miR‑146a‑5p的核苷酸序列如SEQ ID NO:1所示。 3.根据权利 ...
参考价咨询 型号 品牌 MedChemExpress 所属地区美洲 原产地国外-国外 产品摘要 官网链接:https://www.medchemexpress.cn/hsa-mir-146a-5p-mimic.htmlhsa-miR-146a-5p mimic 是一种化学合成的miRNA模拟物,能模拟内源性miRNA,上调miRNA活性,用于功能获得性(gain-of-function)研究。
binding to cirCCT2. miR-146a-5p acts as a tumor suppressor in a variety of cancers, such as glioblastoma (GBM) [20] and lung cancer [21]. We further predicted that the interleukin-1 receptor-associated kinase 1 (IRAK1) was a downstream mRNA of miR-146a-5p through bioinformatics ...
FULL PAPER British Journal of Cancer (2016) 115, 1548–1554 | doi: 10.1038/bjc.2016.367 Keywords: ESCC; miR-146a-5p; Notch2; EMT; invasion; western blotting; qRT-PCR miR-146a-5p mediates epithelial–mesenchymal transition of oesophageal squamous cell carcinoma via targeting Notch2 Cong Wang1,...