www.nature.com/scientificreports OPEN received: 04 July 2016 accepted: 06 September 2016 Published: 23 September 2016 Predicted structure of a Minus-C OBP from Batocera horsfieldi (Hope) suggests an intermediate structure in evolution of OBPs Zhi-Chuan Zheng*, Dong-Zhen Li*, Aiming Zhou...
Odorant binding proteins (OBPs) transport hydrophobic odorants from the environment to odorant receptors and play an important role in specific recognition of volatiles. Here, we expressed and purified a minus-C OBP, BhorOBPm2, from Batocera horsfieldi, a major pest of Popolus, to determine its...
Minus-C气味结合蛋白荧光竞争结合为了更好地了解昆虫气味结合蛋白(odorant binding proteins,OBPs)在桔小实蝇Bactrocera dorsalis嗅觉识别中的作用,并明确其与寄主挥发物的结合特性,本研究克隆了一个"Minus-C"OBP基因Bdor OBP1a.BdorOBP1a基因长480 bp,编码159个氨基酸,其N端含有26个氨基酸的信号肽,去信号肽氨基酸...
故命名为Minus- OBP.RT-PCR结果表明BhorMinus-OBP1,2和3在云斑天牛头部(去除触角)没有表达,在其余9种不同组织中均有表 达,BhorMious-OBP4在触角,前足,中足,后足,中腹和腹末均有表达,但是在下颚须,下唇须和头部则没有表达.Real-time PCR结果表明BhorMious-C OBPs表达水平与云斑天牛性别,日龄及交配与否...
Odorant binding proteins (OBPs) are believed to bind and carry hydrophobic active compounds from the environment to the surface of olfactory receptor neurons. To investigate the potential functional role of the highly expressed XaffOBP9 in binding with semiochemicals, we clon...
Amino acid alignment of (A) Classic OBPs, (B) Minus-C OBPs, and (C) and CSPs.Wu, ZhongzhenBin, ShuyingHe, HualiangWang, ZhengbingLi, MeiLin, Jintian
We report here the first 3D structure of a member of the C-minus class OBPs, AmelOBP14, characterized by only two disulfide bridges of the three typical of classical OBPs. We show that AmelOBP14 possesses a core of 6 伪-helices comparable to that of classical OBPs, and an extra ...
A deeper look at the structures reveals significant differences at their C-termini with functional implications. PBPs from Lepidoptera, those of Bombyx mori (Bmor) or Apol have the longest sequences among classical OBPs. Their C-terminus segment is ∼15 residues long, adopts an α-helical ...
Molecular cloning and functional identification of a Minus-C odorant binding protein from the rice striped stem borer, Chilo suppressalis ( Lepidoptera: Py... Odorant binding proteins(OBPs) play important roles in the perception of host plant volatiles,but Minus-C OBPs(with only four conserved cy...