作为制定实验手术方案的起点,可以参考 Shuman 实验室的 Susie Feng 在 2021 年微型显微镜研讨会上发布的视频《Grin Lens & Baseplate Surgery》,该视频介绍了为小鼠植入 GRIN 镜头和安装底座基板以使用 UCLA Miniscope v4 对其 CA1 区域进行成像的过程。 最后,请确认您拥有兼容的数据采集硬件。如果没有,请获取兼容的...
First, a target brain region is labeled with virus expressing GCaMP6; second, a GRIN lens is implanted above the target brain region; third, following mouse surgical recovery, a miniscope is mounted on the head of the mouse above the GRIN lens; and finally, neural activity is recorded from...