Expression of CSC markers LGR5, CD44, and PROM1 in stemCO and of differentiation markers KRT20, CDX1, and CDX2 in diffCO was further confirmed by quantitative real-time PCR (RT-PCR) (Fig. 2g). Finally, a colony formation assay with single cells prepared from each type of culture ...
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500 μl of diluted serum was incubated with the beads for 1 h at 37 °C on a rotator. Next, the serum was transferred to the second tube of beads for the second round of deple- tion. Finally, the depleted serum was stored at 4 °C for Table 1 Monoclonal antibody characterization ...
For each round of selection, phages (1 × 1011) were applied to a 96-well plate precoated with anti-rTRAP1 antibodies (10 μg/well). The level of specific phage enrichment was calculated by ratio of input and output as described (8). After three rounds of biopanning, positive phage ...