Milk Street Tuesday Nights “offers the home cook a new beginning and a break from a past when fast food meant bad food.” My family have told me on more than one occasion that they'd rather eat at home than at any restaurant. Sometimes we go out to eat and feel a little disappointe...
6/20/19 Port Washington, NY Landmark on Main Street 6/19/19 New Haven, CT College Street Music Hall 6/18/19 Pittsburgh, PA Mr. Smalls Theatre The Milk Carton Kids Sad Songs Comedy Hour 6/13/19 Los Angeles, CA Largo NPR's Mountain Stage 6/9/19 Charleston, WV Mountain Stage ...
Audio: Bob Dylan, Tom Petty & The Heartbreakers – Australia 1986 – Full Show –‘Positively 4th Street, ‘I’m Alright, Ma’ & Many More October 2, 2014 Video: Lucinda Williams Rocks New Song, ‘Protection,’ on ‘Fallon’ – Watch Right Now! October 2, 2014 Audio: Bob Dylan’...
The paradox of choice suggests that an abundance of options actually requires more effort to choose and can leave us feeling unsatisfied with our choice.
Cells were harvested after 18 h, diluted in sterilized PBS buffer to ~ 105 CFU/mL and 5 µL were inoculated into each well of a 96 well plate. Dried peptides (Gel fraction of P:A (60:40) gels at pH 7 and at pH 9 respectively) were re-suspended in 0.1% [v/v] aqueous TFA ...
For protein concen- tration determination, cells were lysed for 30 min on ice in the 96-well plate with 40 L/well lysis buffer consisting of 1% Nonidet P-40, 150 mM NaCl, 1ϫ PBS, 20 mM EDTA, 20 mM EGTA, 4 mM Na3VO4, and 40 mM NaF. After centrifugation, 5 L/well ...
HS-MEG-OH was used to functionalize any possible exposed gold on the crystal surface, while ethanolamine neutralized activated carboxyl groups that did not react with aptamer. Once the final PBS wash reached a stable baseline following the aptasensor functionalization, whole milk was flowed (250 μ...
A Formvar-coated copper grid (Electron Microscopy Sciences, Hatfield, PA, USA) was gently placed on the top of each drop for about 20 min in a humidified chamber. Grids were then washed in PBS and distilled water. Finally, they were contrasted with 2% uranyl acetate for 5 min and air ...