Toru Sakamoto4, Nobuyuki Hizawa4, Tadao Arinami1 and Emiko Noguchi1,3 The recently identified cell surface immunoreceptor MILR1 (mast cell immunoglobulin-like receptor 1; synonyms, Allergin-1) has been shown to suppress immunoglobulin E (IgE)-mediated, mast cell-dependent responses in both mice...
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sRNA was isolated using the miRcute miRNA Isola- tion Kit (Tiangen) according to the manufacturer's proto- col. The template was polyadenylated, and reverse transcribed to cDNA using the Poly (A) miRNA cDNA Synthesis Kit (Invitrogen) according to the manufacturer's instructions. MilRNAs were...
17、s12:将包含0.1%碘化丙啶的0.2×ssc缓冲液加入经s11处理的样品管中,室温,300rpm清洗5min; 18、s13:用0.2×ssc缓冲液漂洗经s12处理的样品,去色。 19、在上述方案的基础上,所述的经lna和fam修饰后的探针的序列如seq id no:3所示。 20、一种苹果斑点落叶病真菌跨界传递致病的milrna的靶基因(命名为md-pe...