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Movie streams are taken with a 488 nm laser at an exposure time of 200 ms or 250 ms done in Ultra-Quiet mode to obtain high temporal resolution. No binning was used; the pixel size for the 60 × objective was 0.108 µm. Microfabrication of microfluidic device The ...
Show full outline Cited by (48) Figures (14) Show 8 more figures Tables (1) Table Extras (7) Download all Movie S1. Migration of E13.5 WT or cKO CCP1 cINs on Homochronic Mixed Cortical Feeder, Related to Figure 1 Movie S2. Migration of pLifeAct-Ruby Electroporated E13.5 WT or...
Download: Download full-size image Figure 6. VIFs Stabilize Microtubule Network Organization and Enhance Cell Polarity in Directed Cell Migration (A) Wound healing response over 200 min in control, vimentin-knockdown, and nocodazole-treated RPE cells as indicated (Movie S12). Scale bar, 200 μm...
Full size image We further assessed the role of mTORC1 on differentiation by quantifying key molecular markers of neutrophils. Here we used flow cytometry to measure the induced surface expression of CD11b, an early differentiation marker also known as integrin αM (ITGAM), and the fMLF receptor...
2A, B, Supplementary Movie 1). Since the measured margin-to-front distance depends not only on mesodermal progression but also on margin progression, we ensured that the observed defect was not reflecting a defect in epiboly. First, we analysed the progression of epiboly in control and nhsl1b...
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With latency, bandwidth, scale and service velocity as the new currencies Gearing up for a 5G Connected World Technology 3G 4G 5G Connecting humans Latency 300ms High Bandwidth 1hr 3GB movie download Scale Millions of devices Source: Accenture Analysis, OpenSignal Connecting humans+devices Connecting...
Full size image We then explored whether Lpd promotes cancer cell invasion during metastasis. In fixed samples, tumors generated from control shRNA LM2 cells prominently invaded the surrounding stroma. However, tumors from Lpd knockdown cells were markedly less invasive (Figure 2e). We investigated...
Full size image Physical barriers and cell confinement A cell may be confined laterally or even from all sides by a dense, impenetrable ECM or tightly adhering cells. Such confinement limits the extension of lateral protrusions; but if a path or tunnel is available within this confined space, ...