In some experiments, the culture medium also contained the EDNRB antagonist, BQ-788 (Sigma-Aldrich, Castle Hill, NSW, Australia; 20 μM), which was added three to four hours prior to the commencement of imaging. Co-cultures were also set up in which segments of small intestine from E11.5...
all of the cells migrate as a collective from one location to another. However, there are also migratory cell populations that must populate the areas through which they migrate, and thus some cells get left behind while others advance.