of multiplex primers included eight primers each for the forward and reverse primers, resulting in 8 × 8 = 64 combinations of primers (Table 1). Although the number of amplified regions depends on the target genome and PCR conditions, thousands of regions were amplified from a wid...
The volume of the PCR reaction mixture was 7 μ l, containing 1 μ l of template DNA, 0.2 μ M of each 1st PCR primers, 3.5 μ l of 2 × Multiplex PCR Buffer (Multiplex PCR Assay Kit Ver.2, Takara Bio, Kusatsu, Japan), and 0.035 μ l of ...