MIG-seq primer set-2 was used to create a different library from the same sample set (Supplementary Table 1). The volume of the PCR reaction mixture was 7 μl, containing 1 μl of template DNA, 0.2 μM of each 1st PCR primers, 3.5 ...
By substituting a segment of the simple sequence repeat sequence in the MIG‐seq primer set (MIG‐seq being a PCR method enabling library construction with low‐quality DNA) with degenerate oligonucleotides, we introduced variability in detectable polymorphisms across various crops. This innovation, ...
MIG-seq amplification of mucus-derived DNA was successful, albeit resulting in shorter amplicons relative to those derived from fin samples. Approximately 14,000 SNPs were identified from both DNA sources, with~50% of the SNPs in common. Phylogenetic analysis using UPGMA clustering indicated that ...
MIG-seq: an effective PCR-based method for genome-wide single-nucleotide polymorphism genotyping using the next-generation sequencing platform 来自 NCBI 喜欢 0 阅读量: 182 作者:Y Suyama,Y Matsuki 摘要: Restriction-enzyme (RE)-based next-generation sequencing methods have revolutionized marker-assisted...
www.nature.com/scientificreports OPEN MIG-seq: an effective PCR- based method for genome-wide single-nucleotide polymorphism received: 19 June 2015 genotyping using the next-accepted: 22 October 2015 Published: 23 November 2015 generation sequencing platform Yoshihisa Suyama & Yu Matsuki ...
本项目创始科学家团队在基础医学研究领域拥有丰厚实战经验,以NGS及RNA-SEQ基因检测技术为基础,通过对海量研究文献深入整理,归纳,再挖掘,再分析;依靠研究团队多年来对细胞信号通路,肿瘤免疫,细胞治疗等研究领域的深刻理解,全面构建自有癌症相关基因与靶向药数据库及癌症知识图谱,帮助临床医师为患者提供最可靠的靶向治疗方案...
DDR3 MIG IP核解决方案 信号方向描述app_addr [ADDR_WIDTH - 1:0]输入该输入指示当前请求的地址。app_cmd [2:0]输入该输入选择当前请求的命令。app_en输入这是app_addr [],app_cmd [2:0],app_sz和app_hi_pri输入的高
brew tap mikessh/repseq brew install migmap-macos # or migmap-linux Another option is to intall MIGMAP using BIOCONDA, see corresponding recipe. MiGMAP can be compiled from sources using Gradle with gradle build. Note that in order for tests to pass IgBlast binaries should be in $PATH var...
self._sin_cached[:seq_len, ...].to(dtype=x.dtype), ) def yarn(self, device): Member gante May 27, 2024 Suggested change def yarn(self, device): def compute_yarn_scaling(self, device): (Or a similar name. Let's use descriptive function names :) ) src/transformers/models...
SeqThr 23-Thr125 TagC-6His FormulationFreeze-dried powder Storage/StabilityAliquot and store at -20℃ to -80℃ for up to 6 months. Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose; buffer containing 50% glycerol is recommen. ...