Confocal Fluorescence Lifetime Imaging Microscopy (FLIM) at the Single Molecule Level. Single Mol. 1, 215–223 (2000). 54. Pires, L., Nogueira, M. S., Pratavieira, S., Moriyama, L. T. & Kurachi, C. Time-resolved fluorescence lifetime for cutaneous melanoma detection. Biomed. Opt. ...
the calculated N 1s–C 1s energy separation for the single N 1s level by about 0.4 eV (with Cth5e9NKorahdni-cSahl aism11ei6g.1en0v eaVl,uiems paplypinrogxtihmaatttihneg interaction in the dimer the initial state energies lowers the showing a slightly larger difference of 0.7...
In Fig. 1d, the current-voltage (I-V) characteristic of a pristine state device (PRI) is shown; note the extremely low conductance level. The device is subsequently electroformed (inset of Fig. 1e) before being switched to LRS (Fig. 1e main). TXM-NEXAFS measurements were ...
Fluorescence spectroscopy is a non-invasive technique which has been extensively used for metabolic imaging in mammalian cells. Fluorescence lifetime imaging microscopy (FLIM), in particular, is a powerful label-free method to probe the local environment and molecular conformation of endogenous fluorophore...
This operational mode is the most suitable for flat and rigid surfaces such as crystal, hard polymers and tissue, enabling the highest resolution level. J. Funct. Biomater. 2017, 8, 7 4 of 20 In this mode, it is possible to capture image artefacts related to a not-flat surface or to ...
These applications open new avenues towards a better interpretation of the spatial organization and treatment responses of functionally relevant molecules, at the single cell level, in normal and cancer cells, offering new potentials for individualized medicine. Keywords: fluorescent nano-probes; super-...
Raman spectrometer coupled with a charge-coupled device (CCD) of the camera was used to visualize, image, map, and collect the Raman spectra of the cells. Raman microspectroscopy (RMS) was used to evaluate NO release at the single-cell level. Raman microspectroscopy was used to study the ...
International Journal of Molecular Sciences Article 3D Visualization of Developmental Toxicity of 2,4,6-Trinitrotoluene in Zebrafish Embryogenesis Using Light-Sheet Microscopy Juneyong Eum 1,†, Jina Kwak 1,†, Hee Joung Kim 2,†, Seoyoung Ki 1, Kooyeon Lee 1, Ahmed A. Raslan 1, Ok ...
Fluorescence lifetime imaging microscopy (FLIM) is a standard technique for probing tiny emission lifetime differences in microscopy samples [22], and both commercial and research-level devices with very high temporal (close to few picoseconds) [22] and spatial resolution are now available. High ...
Thanks to the ability of AFM to sense and apply forces with high accuracy, AFM-based force spectroscopy (AFM-FS) has become an excellent tool to study molecular interactions at the single molecule level [43]. Therefore, during recent decades AFM-FS has been a suitable technique to perform ...