Combining the techniques allows users to harness the high-resolution, large field of view and accessibility of an optical microscope, and the infrared data allows computational analysis without the need for dyes or stains that can damage tissues. Software is able to re-create different stains or ...
a light microscope consists of an optical system with a tube with lenses on each tip: the ‘ocular’ near the eye and the ‘objective’ near the sample. Generally, several objectives are available to perform small (4
embedding and sectioning became possible, there were no adequate substitutes for the numerous specific histological stains generated by the dyestuff industry. Metal staining, although mostly unspecific, revealed cellular fine structures (especially membranes) of great interest in cell biology, although resolu...
However, most biological samples are essentially transparent, and nearly invisible when viewed using a standard optical microscope2. It is therefore highly desirable to be able to produce coloured images without needing to add any stains or dyes, which can alter the sample properties. Here we ...
stains to fluoresce. In modern microscopy, lamps in the range from deep blue to near UV are more often used for this purpose. However, the interest in UV led to the recognition that theelectron beamcould be used as an illuminant of very short wavelength, and it was this that gave rise ...
The gold standard technique requires the addition of dyes or stains to human tissue biopsies. This enables pathologists to see the shapes and patterns of the cells under a microscope and distinguish cancerous tissues from healthy ones. However, even for highly trained readers, such diagnostics can...
The cell has different parts, and those that can absorb stains or dyes are referred to as chromatic. Having absorbed the stain, these parts of the cell become more visible under the microscope and can therefore be easily distinguished from other parts of the same cell. ...
Our Molecular Probes™ FocalCheck™ fluorescent microspheres are specifically designed for examining the alignment, sensitivity, and stability of confocal laser scanning microscopes. Several different multicolored configurations of fl...
However, most biological samples are essentially transparent, and nearly invisible when viewed using a standard optical microscope. It is therefore highly desirable to be able to produce coloured images without needing to add any stains or dyes, which can alter the sample properties. Here we ...
(F) A 3-D reconstruction of the eye of a stage 35Xenopusembryo, fixed in methanol and stained with antibodies to acetylatedα-tubulin, which stains “stable” MTs (Pipernoet al., 1987; Schulzeet al., 1987; Webster and Borisy, 1989). View is from behind the eye. ON denotes the ...