Non-parametric Mann-Whitney test was used for statistics.Results: The alcohol, APAP, TLR9/TLR4 and CCL4, -induced liver injury models all resulted in ALT increase and more important, in increased serum/plasma miR-122 levels compared to control mice. There was a linear correlation between miR-...
preventing degradation by Xrn1, seems to dominate in these constructs, probably due to their excessive length. However, none of the replicons harboring a mutant UTR sequence replicated to significant levels in the presence of miRWTor miRmut(Fig.2e), demonstrating that both functions of miR-122 ...
Our results showed that CPEB1 mRNA (Figure 2b) and protein (Figure 2c) expression levels were significantly increased in the corneal autograft group. We also overexpressed miR-122 in the TKE2 mouse corneal epithelial cell line and measured CPEB1 expression using real-time PCR and western ...
The inhibition ofmiRNA-122resulted in slightly, but significantly, reduced plasma cholesterol levels, mirroring findings from numerous studies in mammalian models [9–11,30]. In contrast to the identification of a decrease in cholesterol biosynthesis genes in these studies, which were considered to b...
A catalytic-site mutant ADAR1 also decreased HBV RNA levels, whereas another adenosine deaminases that act on the RNA (ADAR) family protein, ADAR2, did not. Moreover, the induction of ADAR1 by stimulation with IFN-α also reduced HBV RNA levels. Decreases in endogenous ADAR1 expression by...
MiR-122-5p regulated the expression of proteins associated with cell cycle and apoptosis as well as migration and invasion.a,bThe expression levels associated with cell cycle and apoptosis were determined in PDAC cells using qRT-PCR and western blot after transfection with miR-122-5p mimics.c,d...
The inhibition ofmiRNA-122resulted in slightly, but significantly, reduced plasma cholesterol levels, mirroring findings from numerous studies in mammalian models [9–11,30]. In contrast to the identification of a decrease in cholesterol biosynthesis genes in these studies, which were considered to b...
A few recent studies involving models of heart failure have shown that pathogenic effects of microRNAs (miRNAs) are attributed to their regulatory actions on cardiac mitochondria.#Hypothesis:Inspired by this literature, we set out to test the hypothesis that cardiac dysfunction in PE is mediated by...
(c) Levels of miR214 and CUX1 RNA in Hs578T cells infected with an empty vector or a lentiviral vector constitutively expressing CUX1 shRNA. Data represent the mean ± s.d. of three independent experiments. *P < 0.05 (t-test). (d) Hs578T cells were infected with a ...
Paired tumor and distal normal liver tissue specimens, as well as HBx-LINE1 overexpressing hepatic cells, were used to test the relationship between HBx-LINE1 and miR-122. Levels of HBx-LINE1 and miR-122 were assayed by qRT-PCR and Northern blot. HBx-LINE1-miR-122 binding was analyzed ...