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microRNA与RISC结合，通过与目标mRNA部分互补，导致翻译抑制或mRNA稳定性降低。它
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本文介绍了 mRNA、小干扰核糖核酸(small interfering RNA, siRNA), 小分子核糖核酸(micro RNA, miRNA) 和反义寡核苷酸(antisense oligonucleotide, ASO) 的作用机制及应用现状, 总结并阐释了包括化学修饰、偶联配体和纳米载体在内的多种体内递送策略, 以期为发展更高效安全的RNA 体内递送技术提供理论依据和设计思路。
随着对小RNA的研究的逐渐深入,小RNA的分离逐渐成为一种需求,百泰克利用雄厚的研发实力,开发出高效快捷的小RNA分离纯化试剂盒,对各种不同来源的样品(动物、植物、细胞等)均取得满意的效果。 miRNA提取试剂盒采用*的结合洗脱技术,对小RNA,包括microRNA(miRNA)、small interfering RNA(siRNA)、small nulcear RNA(snRNA)...
小干扰RNA(small interfering RNA,siRNA)是RNA干扰的引发物,激发与之互补的目标mRNA沉默,对基因调控及疾病治疗有重要意义。siRNA作为药物需要克服血管屏障、实现细胞内吞及溶酶体逃逸,同时还需要避免核酸酶作用下发生降解。因此,设计合适的纳米载体以帮助siRNA成功递送进细胞并发挥作用是目前siRNA药物发展的重要目标。纳米...
MicroRNAs (miRNAs) and small interfering RNAs (siRNAs) are 21- to 25-nucleotide RNA molecules that influence their much bigger relatives, the messenger RNAs (mRNAs). Over the past few years, these small RNA species have captivated the study of gene regulation and modified our notions about how...
Small RNAs (sRNAs) including microRNAs (miRNAs) and small interfering RNAs (siRNAs) serve as core players in gene silencing at transcriptional and post-transcriptional levels in plants, but their subcellular localization has not yet been well studied, th
Zeng Y, Wagner EJ, Cullen BR (2002) Both Natural and Designed Micro RNAs Can Inhibit the Expression of Cognate mRNAs When Expressed in Human Cells. Molecular Cell 9: 1327-1333. Zeng Y, Yi R, Cullen BR (2003) miRNAs and small interfering RNAs can inhibit mRNA expression by similar mecha...
这种方法可以快速制备各种标记(同位素、非放射性标记均可)的小于100nt的小分子RNA探针,适用于包括RPAs,Northerns 和原位杂交等各种方法检测小分子核仁RNA( small nuclear RNA,snRNA),small interfering RNA (siRNA),,micro RNA (miRNA)和 mRNA。非放射性标记的探针还可以用于原位杂交研究miRNA或者mRNA在组织中的分布...