Microglia in Glia-Neuron Co-cultures Exhibit Robust Phagocytic Activity Without Concomitant Inflammation or Cytotoxicity.MicrogliaCo-culturesPhagocytosisExcitotoxicityInflammationA simple method to co-culture granule neurons and glia from a single brain region is described, and microglia activation profiles are ...
macrophage precursors (pMacpre) were harvested from differentiation factories and replated onto iPSC-derived MNs in a 1:1 ratio. In parallel, microglia (microglia-like, pMGL) and MNs (pNeuron) were differentiated in monocultures
Co-culture Cell And Tissue Culture Decapitation Animal Euthanization FACS Cell Proliferation Assay ELISA Reagents Antibodies Protein Experimental Models Rattus norvegicus Kits Other Keywords Microglia Astrocytes Neuroinflammation References Streit WJ, Mrak RE, Griffin WS (2004) Microglia and neuroinflammation: a...
7). We further investigated the role of SIRT5 in OGD/R-induced neuronal damage, and a microglia–neuron co-culture system was applied (Fig. 8A). Primary microglial cells were transduced with recombinant adenovirus carrying the SIRT5 coding sequence or shRNA sequence. Frist, the overexpression ...
Subsequently, NLRP3 inflammasome-active microglia induces profound neuronal death in a microglia-neuron co-culture model. Furthermore, Cx3Cr1-based microglia-specific expression of an active NLRP3 mutant greatly exacerbates motor deficits and dopaminergic neuronal loss of MPTP-treated mice. Taken ...
(F) Staining of a neuron exposed to α-syn aggregates analyzed by patch-clamp in co-culture with microglia. (G) Burst frequency measured by patch-clamp in naive neurons (Ctrl), neurons treated with α-syn aggregates (α-syn), and α-syn-treated neurons co-cultured with microglia (α-...
Maresin1 Aβ42 in Neuron-Microglia Co-Culture Models Promoted inflammation resolution by inhibiting chemotaxis and regulating activation of microglia. [203] 2 SMCs relieve microglia-mediated neuroinflammation Quercetin LPS-induced BV2; Aβ-injected wistar rats Inhibited NF-κB pathway; Activated Nrf2/HO...
Activated microglia can affect brain function by pruning neuron synapses and regulating synaptic plasticity [42]. According to the Golgi staining, dendritic length, branch points, and dendritic complexity of neurons in the hippocampal CA1, CA2, CA3, and DG regions were all decreased in IUGR rats ...
Deficient neuron-microglia signaling results in impaired functional brain connectivity and social behavior. Nat. Neurosci. 17, 400–406 (2014). CAS PubMed Google Scholar Sipe, G. O. et al. Microglial P2Y12 is necessary for synaptic plasticity in mouse visual cortex. Nat. Commun. 7, 10905 ...
5 Additional neuron microglia co-culture experiments. a, Representative microscopy images of primary mouse neurons cultured with or without microglia (MG) and then treated with EtOH, POVPC (50 µM), or POVPC with CytoD for 24 h, labeled with DAPI, Tuj1, CD68, and E06. b, ...