To interrogate the full spectrum of chromatin folding ranging from the level of nucleosomes to full chromosomes in mammals, we developed an enhanced 3C-based method called Micro-C. The protocol employs Micrococcal nuclease (MNase) to fragment the genome, which overcomes the resolution limit of ...
路由信息协议 (RIP) 是一种距离矢量路由协议。RIP 的下一代应用安全网关实现支持 RIP 版本 2(请参阅 RFC 2453)和 RIPng(请参阅 RFC 2080)。 RIP 是为小型 IP 网络设计的,根据跃点计数来确定路由;最佳路由的跃点数最少。RIP 基于 UDP,使用端口 520 进行路由更新。通过将路由限制为最多 15 个跃点,该协...
a Schematic of Micro-C protocol. Nuclei were extracted from plant tissue and then separately cross-linked by formaldehyde (FA) and ethylene glycol bis (succinimidyl succinate) (EGS). Subsequently, chromatin was digested with micrococcal nuclease (MNase), and DNA overhangs were filled with biotin-dN...
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For site-directed mutagenesis, primers (Table S2) were designed by using NEBaseChanger (http://neba- sechanger.neb.com/) and were then used to generate point mutation plasmids (Table S3) with a Q5 Site-Directed Mutagenesis Kit (New England Biolabs) following the manufacturer's protocol...