The tail temperature is measured at the point 1 cm away from the base of the tail. To measure the core body temperature, the telemetry transmitter was implanted in the peritoneal cavity under anesthesia with isoflurane more than 7 days before experiments. The core body temperature was ...
as though every move was a strain . All eyes were on Morty as he inched up the rope at a snail's pace. Morty's tail was barely off the ground when his paws began to slip . His knees shook with fear . He listened for the insults...
Polymerase chain reaction (PCR) of DNA isolated from tail biopsies was used to identify the genotype of each animal. All KO mice are complete knockouts with no remaining receptor activity. Inbred C57BL/6NTac mice, throughout referred to as wild-type mice, served as controls. Mice were housed...
“Well, you ain’t petting no mice while you walk with me. You remember where we’re goin’ now?” Lennie looked startled and then in embarrassment hid his face against his knees. “I forgot again.” “Jesus Christ,” George said resignedly. “Well—look, we’re gonna work on a ranc...
Located in the Southwestern US, these mice have shorter, nearly hairless tails. They have a dark grey-brown back and grey sides, making them fairly easy to spot. The body generally grows to between 2 and 2.5 inches with a tail length of 1.5 to 1.75 inches. ...
Healthy aging has emerged as a crucial issue with the increase in the geriatric population worldwide. Food-derived sulfur-containing amino acid ergothionei
Sudden death may result or subacute illness associated with one or more of the following signs may develop: ruffled fur, hunched posture, motionlessness, and neurological deficits. Mice suspended by the tail display coarse tremors of the head and extremities, culminating in clonic convulsions and ...
combined with buprenorphine. Since isoflurane has no effect on ion channel function, this agent is recommended for the kainic acid-induced seizure model [136]. Ketamine/xylazine along with local anesthesia may also be used for this purpose, though ketamine may delay the onset of seizures due to...
Method1: use iPS cells derived from tail tip fibroblasts of eight-week-old male mice and process like before, BV XRY iPS -> BV XRO iPS -> BV XRXR iPS -> PGCL -> primary oocytes -> GV oocytes -> MII oocytes. Then fertilize it with wild-type sperm in in vitro fertilization (IVF)...
Tail suspension test (TST) Each mouse was suspended with adhesive tape affixed 1–2 cm to the tip of the tail and inverted for 6 min, with its nose 20–25 cm away from the ground. Immobile state was considered as no body movement and passive hanging. The ratio of immobility was quantif...