MIBI-TOF(飞行时间多路复用离子束成像)分辨率低至 260 nm ,对800 μm × 800 μm 的视野进行成像,其灵敏度接近单分子检测,可对福尔马林固定石蜡包埋 (FFPE) 人体组织活检进行常规、稳健的成像。MIBI-TOF可以了解(1)单个细胞的表型如何与它们所包含的多细胞结构的功能相关,(2)以亚细胞分辨率量化跨大片完整组织的...
每个抗体结合一个单一的蛋白质目标。 2)高能离子束扫描穿过组织,释放出二次金属同位素离子,由ToF质谱仪检测和量化。 3)生物信息学分析和访问基于云的MIBItracker软件,便于数据可视化。 优势: 多重检测能力:每个组织切片单次扫描检测多达40个蛋白质标记 真实亚细胞分辨率:低至250 nm的细胞结构可以成像,可分析和解释细胞...
225: In-depth characterization of immune cells in preeclampsia using Multiplexed Ion Beam Imaging by Time-of-Flight (MIBI-TOF)doi:10.1016/j.ajog.2019.11.241Shirley GreenbaumMarc BosseAlex BaranskiZumana KhairMike AngeloAmerican Journal of Obstetrics and Gynecology...
鼻咽癌放射治疗后 99mTc—MIBI SPECT显像的临床价值 维普资讯 http://www.cqvip.com
Abstract An ability to characterize the cellular composition and spatial organization of the tumor microenvironment (TME) using multiplexed IHC has been limited by the techniques available. Here we show the applicability of multiplexed ion beam imaging (MIBI) for cell phenotype identification and analysis...
MIBI, which combines time-of-flight secondary ion mass spectrometry (ToF-SIMS) with metal labeled antibodies to simultaneously image 40+ proteins at subcellular spatial resolution, was used to classify cell populations and their expression of immunoregulatory proteins within 54 melanoma samples. Methods ...