FACS分析使用FITC-结合W6/32(anti-HLA-ABC,Sigma,St Louis,MO)和生物素化抗-28-8-6S(anti-H-2 Kb/Db,BD Biosciences,San Diego,CA)m.Ab在去除了红细胞的、淋巴细胞M-提纯(Tebu-bio,Le Perray en Yvelines,France)的脾细胞上进行流式细胞分析研究。使用PE-标记的CT-CD4抗-小鼠CD4(CALTAG,South San Fr...
The vector was then transformed into DH5a competent bacteria (Invitrogen Life Technologies) and grown on LB agar plates with ampicillin (Sigma-Aldrich, St Louis, MO, USA). Up to 10 clones were sequenced for each individual and a total of 691 clones were analyzed. The probability that an ...
Bursky Center for Human Immunology and Immunotherapy Programs, Washington University School of Medicine, St Louis, MO, USA Elise Alspach, Danielle M. Lussier, Alexander P. Miceli, Wei Meng, Cheryl F. Lichti, Daniele Runci, Jeffrey P. Ward, Matthew M. Gubin, Ruan F. V. Medrano, Cora D....
Diluted blood was overlayed onto lymphocyte separation medium (Histopaque, Sigma-Aldrich, St Louis, MO) and underwent gradient centrifugation for cell isolation. The separated cell fraction was removed and washed twice with HBSS before resuspension in tissue culture medium. Tissue harvest from tumor ...
The Andrew M. and Jane M. Bursky Center for Human Immunology and Immunotherapy Programs, Washington University School of Medicine, St Louis, MO, USA Elise Alspach, Danielle M. Lussier, Alexander P. Miceli, Wei Meng, Cheryl F. Lichti, Daniele Runci, Jeffrey P. Ward, Matthew M. Gubin, Ru...
Prior to RT, all the samples were digested with RNase-free DNase (Sigma, St. Louis. MO. USA), at 70°C for 15 min, to avoid any possible DNA contamination. Reverse transcription (RT) RT was carried out with the Transcript or first strand cDNA synthesis kit (Roche Diagnostics, S.L. ...
In some experiments, these lobes were irradiated with 30 Gy and cultured for 5 days, or cultured in medium containing 1.35 mM 2-deoxyguanosine (Sigma, St Louis, MO) for 5 days before transplantation. After anesthetizing 4- to 6-week-old mice of the respective strains with Ketanest/Rompun...
Author information: Department of Pathology, Washington University School of Medicine, St. Louis, MO 63110, USA.doi:10.1111/j.1600-065X.1996.tb00704.xChristopher A. NelsonNicholas J. VinerEmil R. UnanueJohn Wiley & Sons, Ltd.Immunological Reviews...
10% fetal calf serum (Biofluids, Rockville, MD), 2 mM glutamine, 1% penicillin/streptomycin/fungizone, 10 mM HEPES buffer, 1% nonessential amino acids, 1 mM sodium pyruvate (all from Life Technologies, Chagrin Falls, OH), and 5 × 10–5 M β2-mercapto-ethanol (Sigma, St. Louis, MO)...
摘要: Department of Genetics, Washington University School of Medicine, St. Louis, MO 63110.被引量: 26 年份: 1988 收藏 引用 批量引用 报错 分享 全部来源 求助全文 万方医学 NCBI NCBI ResearchGate EBSCO 查看更多 相似文献 引证文献HLA genotype of patients with severe haemophilia A due to intron ...