以若干等份通过与脂多糖(LPS,10微克/ml)、干扰素-γ(IFN-γ,Roche,40ng/ml)和植物凝血素(PHA,5微克/ml)孵育1天活化,来提高HLA-DR分子的表达。通过流式细胞计数,用FITC偶联的mAb L243(BDImmunocytometry Systems,San Jose,CA,USA)监测HLA-DR分子在细胞表面的表达。在LPS、IFN-γ和PHA的存在下孵育MHH preB1...
在另外的一个实施方案中,本发明提供了用于确定MHC I类单体或修饰了的MHC I类单体相对于它们的推定的MHC结合肽的结合亲和度(degree of binding affinity)的方法,通过在重构条件下,用推定的MHC结合肽和单克隆抗体与至少一种附着到固体表面上的此类变性MHC单体或修饰了的MHC单体进行温育,其中单克隆抗体特异性地结合到...
Sa.62. High Frequency of Long-Range SNP Identity for Founder Chromosomes Across the MHCBaschalE.SteckA.JasinskiJ.EisenbarthG.ingentaconnectClinical Immunology San Diego Academic Press
5 × 106 of the prepared splenocytes were plated into each well of 24-well plate and stimulated with either HPV18 E6 or E7 overlapping peptides (5 μg/ml) or HPV18 E6 or E7 short peptides (1 µg/ml) at the presence of GolgiPlug (BD Pharmingen, San Diego, CA, USA) at ...
San Diego: IEEE Press, 1998. 3844−3849. [8] Toma N, Endo S, Yamada K, Miyagi H. An immune optimization inspired by biological immune cell-cooperation for division-and-labor problem. In: Proc. of the 4th Int'l Conf. on Computational Intelligence and Multimedia Applications. Yokusika: ...
Department of Medicine, University of California, San Diego, CA, USA Michael G. B. Hayes, Max W. Chang & Christopher Benner Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA Teresa Aydillo, Florian Krammer & Adolfo García-Sastre Global Health and Emerg...
Sequencing of the pooled libraries was completed on a NovaSeq6000 (Illumina, San Diego, CA) at Novogene (Cambridge, UK). BD Rhapsody data analysis The FASTQ-files generated from the Rhapsody experiment were uploaded onto the Seven Bridges Genomics online platform together with FASTA-files ...
San Diego, California 92101-4495. USA: Academic; 1998. Google Scholar Dixon B, Van Erp SHM, Rodrigues PNS, Egberts E, Stet RJM. Fish major histocompatibility complex genes: an expansion. Dev Comp Immunol. 1995;19:109–33. Article CAS PubMed Google Scholar Janeway CA, Travers P, Wal...
CD4+ T cells play key roles in a range of immune responses, either as direct effectors or through accessory cells, including CD8+ T lymphocytes. In cancer, neoantigen (NeoAg)-specific CD8+ T cells capable of direct tumor recognition have been extensively
人白细胞抗原(hla)系统(人的主要组织相容性复合物[mhc])是免疫系统的重要组成部分,并且由位于6号染色体上的基因控制。它编码在t细胞上专门将抗原肽呈递给t细胞受体(tcr)的细胞表面分子。(另外参见overview of the immune system)。呈递抗原(ag)的mhc 分子分为两大类:i类mhc分子和ii类mhc分子。