上海玉博生物科技有限公司 主要致力于“Anti-MHC II HLA-DR Monoclonal Antibody, Biotin conjugate Pierce-MA112180”的生产销售。多年的“Anti-MHC II HLA-DR Monoclonal Antibody, Biotin conjugate Pierce-MA112180”生产与销售的经验,与各行业新老用户建立了稳定的合
PerCP/Cyanine5.5-CD45R/B220 (clone: RA3-6B2, Biolegend Cat # 103236, 1:200), PerCP/Cyanine5.5-CD11b (clone: M1/70, Biolegend Cat # 101228, 1:200), PerCP/Cyanine5.5-CD11c Antibody (clone: N418, Biolegend Cat # 117328, 1:200), APC-TCR γ/δ Antibody (clone: GL3, Biolegend...
anti-mouse CD8a Antibody(mCD8a BV711)购自Biolegend,货号100747; BamHI、BglII、EcoNI、SspI酶购自NEB,货号分别为R3136、R0144、R0521、R3132; 实施例1 MHC分子人源化小鼠 本实施例对非人动物(如小鼠)进行改造,使该非人动物体内包含编码人B2M和HLA-A2.1蛋白的核苷酸序列,得到经遗传修饰的非人动物体内可表达人...
The F4/80 (BM8) antibody was obtained from Biolegend (San Diego, CA). Antibodies against CD3e (2C11), CD4 (RM4-5), CD8α (53-6.7), CD11b (M1/70), CD25 (PC61.5), CD86 (24F), Foxp3 (FJK-16S), and TCRβ (H57-597) were from eBioscience. ELISA Analysis of cytokine...
上海玉博生物科技有限公司 主要致力于“PIERCEMA119227Anti-MHC II HLA-DP/DR Monoclonal Antibody100 µg 100 µg”的生产销售。多年的“PIERCEMA119227Anti-MHC II HLA-DP/DR Monoclonal Antibody100 µg 100 µg”生产与销售的经验,与各行业新老用户建立了稳定
For immunohistochemistry (IHC), sections were incubated with SARS-CoV-2 nucleocapsid protein (vNP) antibody (CST, 33717, 1:200) for 30 min. The incubated sections were then labeled with horseradish peroxidase (HRP) and re-stained with hematoxylin before reacting with the chromogenic diaminobenzidine...
MHC-II类肽路径(蓝色):外源性抗原通过内涵体途径被摄取,未成熟的MHC-II蛋白复合物通过固定链(Invariant chain(li))保持稳定,并通过高尔基体释放MHC-II分子,而固定链(Invariant chain(li)在此过程中被处理成短肽(CLIP),CLIP之后会被和MHC-II分子结合的肽交换,最终形成成熟的MHC-II肽复合物被运送至细胞表面。β...
Ag-BCR—class II complexes were isolated as detailed above. MHC class II molecules were released from Ag-BCR complexes by incubation in TNE and 1.0% Triton X-100 for 2–4 h at room temperature. Released I-Ak class II molecules were then immunoprecipitated with either 11-5.2 (BioLegend, ca...
After 48 h stimulation, cells were washed with PBS, and collected cells in FACS buffer (2% FBS/PBS) were stained with either PE-conjugated isotype control (BioLegend) or PE-conjugated anti-human HLA-A, B, C antibody (BioLegend). After 30 min incubation on ice, cells were washed ...
上海玉博生物科技有限公司 主要致力于“PIERCEMA127175Anti-MHC II HLA-DR beta Monoclonal Antibody100 µg4667 100 µg”的生产销售。多年的“PIERCEMA127175Anti-MHC II HLA-DR beta Monoclonal Antibody100 µg4667 100 µg”生产与销售的经验,与各行业新老用